Engineering a better light sheet in an axicon-based system using a flattened Gaussian beam of low order
- PMID: 35104051
- DOI: 10.1002/jbio.202100342
Engineering a better light sheet in an axicon-based system using a flattened Gaussian beam of low order
Abstract
Lasers are fundamental tools in research and development. The shape of an incident laser beam directly affects the results, when it propagates through complex structured meso-aspheric optical elements. In conic-based systems utilizing elements such as axicons, the impact of secondary lobes is mostly overlooked, although the intensity distributions at the central spot and the side-lobes directly affect the beam properties. We investigate the interaction of two axicons (160° and 170°) with incident beams approximated by Gaussian, high-order Flattened-Gaussian, and low-order Flattened-Gaussian functions. We demonstrate that replacing an incident Gaussian beam with a low-order Flattened-Gaussian beam reduces the secondary lobes and significantly improves the uniformity of the intensity profile. We practically applied this effect in engineering a conic-aspheric-based static light-sheet microscope producing markedly improved results.
Keywords: flattened Gaussian beam; laser beam shaping; meso-aspheric optics; static light-sheet microscopy.
© 2022 The Authors. Journal of Biophotonics published by Wiley-VCH GmbH.
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References
REFERENCES
-
- M. Fred, Opt. Photonics News. 2003, 14, 30.
-
- F.M. Dickey, Laser beam shaping techniques, (2004) 338-348. https://doi.org/10.1117/12.407361.
-
- L. M. Soroko, Meso-Optics: Foundations and Applications, World Scientific, Singapore 1996.
-
- V. Peet, Laser beam shaping by conical refraction in biaxial crystals, 8429 (2012) 842914. https://doi.org/10.1117/12.922201.
-
- J. H. McLeod, J. Opt. Soc. Am. 1954, 44, 592. https://doi.org/10.1364/josa.44.000592.
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