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. 2022 Jan;10(2):e15163.
doi: 10.14814/phy2.15163.

Plasma miR-21 as a potential predictor in prediabetic individuals with a positive family history of type 2 diabetes mellitus

Zakieh Yazdanpanah  1 , Nasrin Kazemipour  1 , Seyed Mehdi Kalantar  2 , Mohammad Yahya Vahidi Mehrjardi  3
Affiliations

Plasma miR-21 as a potential predictor in prediabetic individuals with a positive family history of type 2 diabetes mellitus

Zakieh Yazdanpanah et al. Physiol Rep. 2022 Jan.

Abstract

Type 2 diabetes mellitus (T2DM) is a heritable metabolic perturbation, rapidly growing across the world. Primary recognition of susceptible individuals with a family history of type 2 diabetes (FHD) in the prediabetes stage could delay the onset of T2DM or reduce complications induced by diabetes. This study aims to evaluate the expression levels of miR-21, miR-126 as noninvasive predictive biomarkers in individuals with genetic predisposition and investigate the correlation of miRNAs and cardiometabolic risk factors. Our study demonstrated that miR-21 expression has a notable elevate in both groups of T2DM and pre-T2DM. miR-21 expression was distinguished in the pre-T2DM and T2DM from the nondiabetic individuals by ROC curve analysis with AUC of 0.77 (95% CI 0.65-0.90; p = 0.0004) and AUC of 0.78 (95% CI 0.64-0.92; p = 0.0042), respectively. The relative gene expression of miR-126 was nearly equal among groups. miR-21 expression was positively associated with glycosylated hemoglobin (HbA1c), fasting blood sugar (FBS), and triglyceride (TG) and might have diagnostic value for T2DM and pre-T2DM. This study has revealed that the expression level of miR-21 can be considered as a non-invasive and rapid tool for distinguishing pre-T2DM and T2DM counterparts from healthy individuals.

Keywords: family history of type 2 diabetes mellitus; microRNA-126-3p; microRNA-21; prediabetes.

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Conflict of interest statement

The authors do not have any conflict of interest including any financial, personal, or other relationships with other people or organizations.

Figures

FIGURE 1
FIGURE 1
Relative expression of miR‐21 (a) and miR‐126 (b) in the plasma samples of healthy, pre‐T2DH, and T2DH determined by RT‐qPCR. After the data were normalized with SNORD47 (as an internal control), the expression of miR‐21 and miR‐126 was calculated by fold change. The data were presented as mean ±SD and analyzed by one‐way ANOVA (Bonferroni's multiple comparisons test). miR‐21 expression was significantly increased in pre‐T2DM and T2DM patients compared with the healthy group, while no significant difference was observed between pre‐T2DM and T2DM groups. There was no statistically significant difference between the three groups regarding the expression of miR‐126. Bars represent mean. Error bars represent SD
FIGURE 2
FIGURE 2
Receiver operating characteristic (ROC) curves for the capacity of the plasma miR‐21 to evaluate the diagnostic values for pre‐T2DM (a) and T2DM (b). ROC curves demonstrated an approximately equal level of diagnostic performance for both pre‐T2DM and T2DM groups with the healthy group with AUC of 0.77 (95% CI 0.65–0.90; p = 0.0004) and AUC of 0.78 (95% CI 0.0.64–0.92; p = 0.0042), respectively
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