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Review
. 2021 Dec;41(6):1052-1064.
doi: 10.1007/s11596-021-2470-7. Epub 2021 Dec 21.

Recent Developments in SARS-CoV-2 Neutralizing Antibody Detection Methods

Jo-Lewis Banga Ndzouboukou  1 , Yan-di Zhang  1 , Xiong-Lin Fan  2
Affiliations
Review

Recent Developments in SARS-CoV-2 Neutralizing Antibody Detection Methods

Jo-Lewis Banga Ndzouboukou et al. Curr Med Sci. 2021 Dec.

Abstract

The ongoing Coronavirus disease 19 pandemic has likely changed the world in ways not seen in the past. Neutralizing antibody (NAb) assays play an important role in the management of the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) outbreak. Using these tools, we can assess the presence and duration of antibody-mediated protection in naturally infected individuals, screen convalescent plasma preparations for donation, test the efficacy of immunotherapy, and analyze NAb titers and persistence after vaccination to predict vaccine-induced protective effects. This review briefly summarizes the various methods used for the detection of SARS-CoV-2 NAbs and compares their advantages and disadvantages to facilitate their development and clinical application.

Keywords: Coronavirus disease 19; enzyme-linked immunosorbent assay; lateral flow immunoassays; neutralizing antibodies; plaque reduction neutralization test; pseudovirus-based neutralization assays; severe acute respiratory syndrome coronavirus-2; viral neutralization test.

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