Subregions of a conserved part of the HIV gp41 transmembrane protein are differentially recognized by antibodies of infected individuals
- PMID: 3491755
- PMCID: PMC1167260
- DOI: 10.1002/j.1460-2075.1986.tb04605.x
Subregions of a conserved part of the HIV gp41 transmembrane protein are differentially recognized by antibodies of infected individuals
Abstract
A 240-bp DNA fragment encoding a peptide, designated ENV(80), homologous to a conserved part of the gp41 transmembrane glycoprotein of human immunodeficiency virus (HIV) was chemically synthesized and inserted into different plasmid expression vectors. Escherichia coli transformants containing these plasmid constructs produced upon induction high amounts of either an ENV(80) peptide of relative molecular mass (Mr) of 10,000 or the same ENV(80) peptide N-terminally fused to E. coli chloramphenicol acetyltransferase (CAT) or to mouse dihydrofolate reductase (DHFR) having Mr of 36,000 and 31,000 respectively. All polypeptides containing the ENV(80) sequences were strongly reactive with antibodies present in sera from AIDS virus-infected individuals, but not with control sera. The strategy of gene assembly allowed the expression of ENV(80) subfragments fused to DHFR. The serodiagnosis of 15 positive sera by Western blot analysis using these bacterially synthesized ENV(80) subfragments revealed the presence of several immunoreactive epitopes on the 80-amino acid polypeptide which were recognized differently by the various patients.
Similar articles
-
Presence of antibodies to a putatively immunosuppressive part of human immunodeficiency virus (HIV) envelope glycoprotein gp41 is strongly associated with health among HIV-positive subjects.Proc Natl Acad Sci U S A. 1988 Jul;85(14):5225-9. doi: 10.1073/pnas.85.14.5225. Proc Natl Acad Sci U S A. 1988. PMID: 2455899 Free PMC article.
-
Identification of homologous regions in human immunodeficiency virus I gp41 and human MHC class II beta 1 domain. I. Monoclonal antibodies against the gp41-derived peptide and patients' sera react with native HLA class II antigens, suggesting a role for autoimmunity in the pathogenesis of acquired immune deficiency syndrome.J Exp Med. 1988 Mar 1;167(3):914-23. doi: 10.1084/jem.167.3.914. J Exp Med. 1988. PMID: 3127528 Free PMC article.
-
Characterization and epitope mapping of a human monoclonal antibody reactive with the envelope glycoprotein of human immunodeficiency virus.J Immunol. 1987 Dec 15;139(12):4027-33. J Immunol. 1987. PMID: 2447158
-
Monoclonal antibody identifies a highly conserved and immunodominant epitope of the human immunodeficiency virus transmembrane protein.Hybridoma. 1988 Apr;7(2):117-28. doi: 10.1089/hyb.1988.7.117. Hybridoma. 1988. PMID: 2453451
-
Comparison of Western blot (immunoblot) based on recombinant-derived p41 with conventional tests for serodiagnosis of human immunodeficiency virus infections.J Clin Microbiol. 1988 Jan;26(1):116-20. doi: 10.1128/jcm.26.1.116-120.1988. J Clin Microbiol. 1988. PMID: 3277988 Free PMC article.
Cited by
-
Subunit-selective mutagenesis indicates minimal polymerase activity in heterodimer-associated p51 HIV-1 reverse transcriptase.EMBO J. 1991 Dec;10(12):3905-11. doi: 10.1002/j.1460-2075.1991.tb04960.x. EMBO J. 1991. PMID: 1718745 Free PMC article.
-
Processing protease and reverse transcriptase from human immunodeficiency virus type I polyprotein in Escherichia coli.J Virol. 1988 Apr;62(4):1433-6. doi: 10.1128/JVI.62.4.1433-1436.1988. J Virol. 1988. PMID: 2450209 Free PMC article.
-
Integral membrane protein P16 of bacteriophage PRD1 stabilizes the adsorption vertex structure.J Virol. 2004 Sep;78(18):9790-7. doi: 10.1128/JVI.78.18.9790-9797.2004. J Virol. 2004. PMID: 15331712 Free PMC article.
-
Presence of antibodies to a putatively immunosuppressive part of human immunodeficiency virus (HIV) envelope glycoprotein gp41 is strongly associated with health among HIV-positive subjects.Proc Natl Acad Sci U S A. 1988 Jul;85(14):5225-9. doi: 10.1073/pnas.85.14.5225. Proc Natl Acad Sci U S A. 1988. PMID: 2455899 Free PMC article.
-
Asp-51 and Asp-120 are important for the transport function of the Escherichia coli melibiose carrier.J Bacteriol. 1992 May;174(9):3083-6. doi: 10.1128/jb.174.9.3083-3086.1992. J Bacteriol. 1992. PMID: 1569035 Free PMC article.
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Miscellaneous
