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Review
. 2021 Nov 22:19:6315-6327.
doi: 10.1016/j.csbj.2021.11.030. eCollection 2021.

M-MuLV reverse transcriptase: Selected properties and improved mutants

Igor P Oscorbin  1 , Maxim L Filipenko  1
Affiliations
Review

M-MuLV reverse transcriptase: Selected properties and improved mutants

Igor P Oscorbin et al. Comput Struct Biotechnol J. .

Abstract

Reverse transcriptases (RTs) are enzymes synthesizing DNA using RNA as the template and serving as the standard tools in modern biotechnology and molecular diagnostics. To date, the most commonly used reverse transcriptase is the enzyme from Moloney murine leukemia virus, M-MuLV RT. Since its discovery, M-MuLV RT has become indispensable for modern RNA studies; the range of M-MuLV RT applications is vast, from scientific tasks to clinical testing of human pathogens. This review will give a brief description of the structure, thermal stability, processivity, and fidelity, focusing on improving M-MuLV RT for practical usage.

Keywords: Fusion proteins; M-MuLV RT; Random mutagenesis; Reverse transcriptase; Site-directed mutagenesis.

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Conflict of interest statement

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

None
Graphical abstract
Fig. 1
Fig. 1
Schematic representation of M-MuLV RT structure (A) and functionally important amino acids residues (B).
See this image and copyright information in PMC

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