miR-301a inhibits adipogenic differentiation of adipose-derived stromal vascular fractions by targeting HOXC8 in sheep

doi:10.1111/asj.13661

. 2021 Jan-Dec;92(1):e13661.

doi: 10.1111/asj.13661.

miR-301a inhibits adipogenic differentiation of adipose-derived stromal vascular fractions by targeting HOXC8 in sheep

Bishi Zhao¹, Yangyang Pan¹, Liying Qiao¹, Jianhua Liu¹, Kaijie Yang¹, Yu Liang¹, Wenzhong Liu¹

Affiliations

PMID: 34856652
DOI: 10.1111/asj.13661

miR-301a inhibits adipogenic differentiation of adipose-derived stromal vascular fractions by targeting HOXC8 in sheep

Bishi Zhao et al. Anim Sci J. 2021 Jan-Dec.

. 2021 Jan-Dec;92(1):e13661.

doi: 10.1111/asj.13661.

Authors

Bishi Zhao¹, Yangyang Pan¹, Liying Qiao¹, Jianhua Liu¹, Kaijie Yang¹, Yu Liang¹, Wenzhong Liu¹

Affiliation

¹ College of Animal Science, Shanxi Agricultural University, Taigu, China.

PMID: 34856652
DOI: 10.1111/asj.13661

Abstract

MicroRNAs (miRNAs) regulate adipogenic differentiation in stromal vascular fractions (SVFs) through post-transcriptional regulation of transcription factors and other functional genes. miR-301 and the homeobox C8 (HOXC8) gene are involved in lipid homeostasis; however, their roles in the adipogenic differentiation of ovine SVFs are unknown. Here, we explored the effects of miR-301 and HOXC8 on adipogenic differentiation in ovine SVFs and the regulatory role of miR-301a in HOXC8 expression. Additionally, we evaluated the effect of miR-301a and HOXC8 on the mRNA abundance of adipogenic markers and the ability of ovine SVFs to accumulate lipids. We found that miR-301a regulates adipogenic differentiation in ovine SVFs by directly targeting the 3'-untranslated region of HOXC8, resulting in significant downregulation of the HOXC8 mRNA and protein. Moreover, miR-301a overexpression suppressed adipogenic differentiation in ovine SVFs and significantly inhibited the expression of adipogenesis-related genes-including adiponectin, C/EBPα, PPARγ, and FABP4. Conversely, HOXC8 overexpression in ovine SVFs increased the accumulation of lipid droplets and remarkably promoted the expression of adipogenic markers. Taken together, our results indicate that miR-301a attenuates the adipogenic differentiation of ovine SVFs by targeting HOXC8. These findings improve our understanding of the mechanism of lipid accumulation and metabolism in sheep.

Keywords: HOXC8; adipogenesis; miR-301a; sheep; stromal vascular fraction.

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[1] Agarwal, V., Bell, G. W., Nam, J. W., & Bartel, D. P. (2015). Predicting effective microRNA target sites in mammalian mRNAs. eLife, 4, e05005. https://doi.org/10.7554/eLife.05005

[2] Agarwal, V., Bell, G. W., Nam, J. W., & Bartel, D. P. (2015). Predicting effective microRNA target sites in mammalian mRNAs. eLife, 4, e05005. https://doi.org/10.7554/eLife.05005

[3] Ambele, M. A., Dhanraj, P., Giles, R., & Pepper, M. S. (2020). Adipogenesis: A complex interplay of multiple molecular determinants and pathways. International Journal of Molecular Sciences, 21, 4283. https://doi.org/10.3390/ijms21124283

[4] Ambele, M. A., Dhanraj, P., Giles, R., & Pepper, M. S. (2020). Adipogenesis: A complex interplay of multiple molecular determinants and pathways. International Journal of Molecular Sciences, 21, 4283. https://doi.org/10.3390/ijms21124283

[5] Bai, W. L., Wang, J. J., Yin, R. H., Dang, Y. L., Wang, Z. Y., Zhu, Y. B., Cong, Y. Y., Deng, L., Guo, D., Wang, S. Q., Yang, S. H., & Xue, H. L. (2017). Molecular characterization of HOXC8 gene and methylation status analysis of its exon 1 associated with the length of cashmere fiber in Liaoning cashmere goat. Genetica, 145, 115-126. https://doi.org/10.1007/s10709-017-9950-5

[6] Bai, W. L., Wang, J. J., Yin, R. H., Dang, Y. L., Wang, Z. Y., Zhu, Y. B., Cong, Y. Y., Deng, L., Guo, D., Wang, S. Q., Yang, S. H., & Xue, H. L. (2017). Molecular characterization of HOXC8 gene and methylation status analysis of its exon 1 associated with the length of cashmere fiber in Liaoning cashmere goat. Genetica, 145, 115-126. https://doi.org/10.1007/s10709-017-9950-5

[7] Bartel, D. P. (2009). MicroRNAs: Target recognition and regulatory functions. Cell, 136, 215-233. https://doi.org/10.1016/j.cell.2009.01.002

[8] Bartel, D. P. (2009). MicroRNAs: Target recognition and regulatory functions. Cell, 136, 215-233. https://doi.org/10.1016/j.cell.2009.01.002

[9] Bertero, T., Lu, Y., Annis, S., Hale, A., Bhat, B., Saggar, R., Saggar, R., Wallace, W. D., Ross, D. J., Vargas, S. O., Graham, B. B., Kumar, R., Black, S. M., Fratz, S., Fineman, J. R., West, J. D., Haley, K. J., Waxman, A. B., Chau, B. N., … Chan, S. Y. (2014). Systems-level regulation of microRNA networks by miR-130/301 promotes pulmonary hypertension. Journal of Clinical Investigation, 124, 3514-3528. https://doi.org/10.1172/JCI74773

[10] Bertero, T., Lu, Y., Annis, S., Hale, A., Bhat, B., Saggar, R., Saggar, R., Wallace, W. D., Ross, D. J., Vargas, S. O., Graham, B. B., Kumar, R., Black, S. M., Fratz, S., Fineman, J. R., West, J. D., Haley, K. J., Waxman, A. B., Chau, B. N., … Chan, S. Y. (2014). Systems-level regulation of microRNA networks by miR-130/301 promotes pulmonary hypertension. Journal of Clinical Investigation, 124, 3514-3528. https://doi.org/10.1172/JCI74773

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miR-301a inhibits adipogenic differentiation of adipose-derived stromal vascular fractions by targeting HOXC8 in sheep

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miR-301a inhibits adipogenic differentiation of adipose-derived stromal vascular fractions by targeting HOXC8 in sheep

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