Healing of colonic anastomosis in rats under obstructive ileus conditions
- PMID: 34849396
- PMCID: PMC8627191
- DOI: 10.15190/d.2021.8
Healing of colonic anastomosis in rats under obstructive ileus conditions
Abstract
Background: The anastomosis leak in colon resections is a crucial post-operative complication with significant morbidity and mortality. Methods: Forty (40) Wistar rats were allocated in two groups. In SHAM group only anastomosis was performed. In ILEUS group anastomosis was performed following one day of ileus. Animals in both groups were subdivided in two groups according to the day they were sacrificed, 4th or 8th post-operative day. A number of variables between the groups were estimated.
Results: Body weight loss was higher following obstructive ileus on both days. Adhesion score in 4th and 8th post-operative day was higher in ILEUS1, ILEUS2 groups compared to SHAM1, SHAM2 groups respectively (p<0.001 for both). Neovascularization decreased following obstructive ileus compared to control on the 4th day (ILEUS1 vs. SHAM1, p=0.038). Bursting pressure was lower in ILEUS2 group than SHAM2 group (p<0.001). The number of fibroblasts decreased following obstructive ileus compared to control on the 4th and 8th day (ILEUS1 vs. SHAM1, p=0.001, ILEUS2 vs SHAM2, p=0.016). Hydroxyproline concentration was decreased in ILEUS2 group compared to SHAM2 group (p<0.001).
Conclusions: The balance of collagenolysis and collagenogenesis plays a decisive role in the healing of anastomoses following bowel obstruction. Under those circumstances, anastomosis' bursting pressure is reduced owning to decreased neovascularization, reduced fibroblast presence and lower hydroxyproline concertation. In our study, local inflammation, neocollagen concentration and collagenase activity were not associated with this adverse effect. However, further research should delineate the mechanisms of healing of colonic anastomoses and identify those factors that can improve our outcomes.
Keywords: Anastomosis; bursting pressure; collagen.; ileus; leak.
Copyright © 2021, Despoudi K et al., Applied Systems and Discoveries Journals.
Conflict of interest statement
Conflict of interests: The authors declare no conflicts of interest.
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References
-
- Progress in rectal cancer staging and treatment. Pramateftakis M G, Kanellos D, Vrakas G, Tsachalis Tau, Raptis D, Makrantonakis A, Koukouritaki Z, Kanellos I. Techniques in coloproctology. 2010;14 Suppl 1:S29–31. - PubMed
-
- Anastomotic leakage following anterior resection for rectal cancer. Kanellos I, Vasiliadis K, Angelopoulos S, Tsachalis T, Pramateftakis M G, Mantzoros I, Betsis D. Techniques in coloproctology. 2004;8 Suppl 1:s79–81. - PubMed
-
- Oncologic impact of anastomotic leakage after low anterior resection for rectal cancer. Mantzoros I. Techniques in coloproctology. 2010;14 Suppl 1:S39–41. - PubMed
-
- Anastomotic leakage after colonic resection. Kanellos D. Techniques in coloproctology. 2010;14 Suppl 1:S43–4. - PubMed
-
- Parameters of healing in approximative intestinal anastomosis. Rygl M, Novotna J, Herget J, Skaba R, Snajdauf J. European journal of pediatric surgery : official journal of Austrian Association of Pediatric Surgery ... [et al] = Zeitschrift fur Kinderchirurgie. 2009;19(1):25–9. - PubMed
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