Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Sep;200(9):4007-4016.
doi: 10.1007/s12011-021-02992-9. Epub 2021 Nov 5.

Alleviating Effect of Methionine on Intestinal Development and Intercellular Junction Induced by Nickel

Bangyuan Wu  1   2 , Yongci Tan  3 , Haiying Huang  3 , Yiwei Liu  3 , Tingrui Bai  3 , Lulu Yang  3
Affiliations

Alleviating Effect of Methionine on Intestinal Development and Intercellular Junction Induced by Nickel

Bangyuan Wu et al. Biol Trace Elem Res. 2022 Sep.

Abstract

The aim of this study was to investigate the alleviating effect of methionine (Met) on intestinal injury induced by nickel. The mice were divided into six groups: Met-deficient + nickel group (MDN), Met-deficient group (MD), Met + nickel group (MN), high-dose Met + nickel group (HMN), high-dose Met group (HM), and blank control group (BC). Histopathological techniques, Alcian blue-periodic acid Schiff (AB-PAS) staining, enzyme-linked immunosorbent assay (ELISA), and real-time PCR were used to study the changes of intestinal development, the number of goblet cells, and the intercellular junction. The results showed that Met can inhibit the intestinal villus length and crypt depth decreases induced by nickel and increase the index villus length and crypt depth (V/C), the number of goblet cells, and the content of diamine oxidase (DAO) and decrease the content of fatty acid binding protein2 (FABP2) and endotoxin (ET) of the intestinal mucosa damage parameters, and the mRNA expression of intercellular junction (occludin, ZO-1, claudin-1) was damaged. It is suggested that Met could help inhibit the toxic effect of nickel on the intestinal development and intercellular connection.

Keywords: Goblet; Intercellular junction; Methionine; Morphological structure; Nickel; Small intestine.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Lu CY, Lu XG, Zou X, Cheng H, Xu Q (2015) Current situation and utilization technology of nickel ore in China. Chinese J Nature 37(4):269–277. https://doi.org/10.3969/j.issn.0253-9608.2015.04.004 - DOI
    1. Liu G, Sun L, Pan A, Zhu M, Li Z, Wang Z, Liu X, Ye X, Li H, Zheng H (2015) Nickel exposure is associated with the prevalence of type 2 diabetes in Chinese adults. Int J Epidemiol 44(1):240–248. https://doi.org/10.1093/ije/dyu200 - DOI - PubMed
    1. Akinwumi KA, Jubril AJ, Olaniyan OO, Umar YY (2020) Ethanol extract of nigella sativa has antioxidant and ameliorative effect against nickel chloride-induced hepato-renal injury in rats. Clin Phytoscience 6(1):64–76. https://doi.org/10.1186/s40816-020-00205-9 - DOI
    1. IARC (2012) A review of human carcinogens, part C: arsenic, metals, fibres and dust. In: IARC monographs on the evaluation of carcinogenic risks to humans, vol. 100C. Lyon: World Health Organization; p. 169–211
    1. Akinci IE, Ongel O (2011) Amelioration of nickel toxicity by humic acid on bean (phaseolus vulgaris) seedling growth. Ekoloji 20(79):29–37. https://doi.org/10.5053/ekoloji.2011.794 - DOI

LinkOut - more resources