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. 1987 Apr;61(4):1253-7.
doi: 10.1128/JVI.61.4.1253-1257.1987.

Methylation as a modulator of expression of human immunodeficiency virus

D P Bednarik, J D Mosca, N B Raj

Methylation as a modulator of expression of human immunodeficiency virus

D P Bednarik et al. J Virol. 1987 Apr.

Abstract

When Vero or murine cells were stably transfected with the human immunodeficiency virus (HIV) long terminal repeat (LTR) that directs the chloramphenicol acetyltransferase (CAT) gene (pU3R-III-CAT), expression was suppressed. Treatment with the nucleoside analog 5-azacytidine (5-azaC) restored CAT expression. S1 nuclease analysis and a nuclear run-on assay demonstrated that activation of the latent HIV LTR by 5-azacytidine occurred at the transcriptional level. Southern blot analysis demonstrated that this activation was due to the demethylation of cytosine residues in the LTR enhancer. Thus, the HIV LTR appears to be susceptible to transcriptional inactivation by methylation, a process that is proposed to play a modulatory role in viral latency.

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References

    1. Science. 1985 Jan 11;227(4683):171-3 - PubMed
    1. Nature. 1987 Jan 1-7;325(6099):67-70 - PubMed
    1. Nucleic Acids Res. 1976 Sep;3(9):2303-8 - PubMed
    1. J Mol Biol. 1977 Jun 15;113(1):237-51 - PubMed
    1. Biochemistry. 1979 Nov 27;18(24):5294-9 - PubMed

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