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. 2021 Aug 30;16(1):56.
doi: 10.5334/gh.943. eCollection 2021.

Circulating MicroRNAs as Biomarkers of Accelerated Sarcopenia in Chronic Heart Failure

Rizwan Qaisar  1 , Asima Karim  1   2 , Tahir Muhammad  3 , Islam Shah  4 , Javaidullah Khan  5
Affiliations

Circulating MicroRNAs as Biomarkers of Accelerated Sarcopenia in Chronic Heart Failure

Rizwan Qaisar et al. Glob Heart. .

Abstract

Background: Sarcopenia is a critical finding in patients with chronic heart failure (CHF). However, the search for a definitive biomarker to predict muscle and functional decline in CHF remains elusive.

Objectives: We aimed to correlate the circulating levels of selected miRs with the indexes of sarcopenia during healthy aging and in patients with CHF.

Methods: We analyzed the association of circulating microRNAs (miRs) levels including miR-21, miR-434-3p, miR424-5p, miR-133a, miR-455-3p and miR-181a with sarcopenia indexes in male, 61-73 years old healthy controls and patients with CHF (N = 89-92/group).

Results: Patients with CHF had lower hand-grip strength (HGS), appendicular skeletal mass index (ASMI) and physical capacity than healthy controls. Circulating miR-21 levels were higher and miR-181a, miR-133a, miR-434-3p and miR-455-3p levels were lower in patients with CHF than healthy controls. Among the sarcopenia indexes, HGS showed the strongest correlation with miR-133a while ASMI showed the strongest correlations with miR-133a, miR-434-3p and miR-455-3p. Among the miRs, miR-434-3p showed the highest area under the curve in testing for sensitivity and specificity for CHF. These changes were associated with higher expressions of the markers of inflammation, oxidative stress and muscle damage in CHF patients.

Conclusion: Taken together, our data show that circulating miRs can be useful markers of muscle health and physical capacity in the sarcopenic elderly with CHF.

Keywords: Sarcopenia; hand-grip strength; heart failure; microRNAs.

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Conflict of interest statement

The authors have no competing interests to declare.

Figures

Figure 1
Figure 1
Relative expressions of the plasma miRs (A) and the markers of inflammation and oxidative stress (B) in the healthy controls (n = 92) and the patients with CHF (n = 89). Values are expressed as mean ± SEM, *p < 0.05 vs. the healthy controls. (Interleukin-10, IL-10; Transforming growth factor-beta 1, TGF-b1; c-c motif chemokine receptor 5, CCR5; c-x-c motif chemokine ligand 8, CXCL-8; Interleukin-6, IL-6; c-x-c motif chemokine ligand 2, CXCL2; adrenomedullin, ADM; superoxide dismutase-1, SOD1; glutathione synthetase, glutathione peroxidase-1 GPX1).
Figure 2
Figure 2
Linear regression analysis of the relationships of plasma miRs with hand-grip strength (HGS) in healthy controls (n = 92) and patients with the CHF (n = 89).
Figure 3
Figure 3
ROC curve illustrating sensitivity and specificity of the plasma miRs in discriminating healthy controls (n = 92) from the patients with CHF (n = 89).
See this image and copyright information in PMC

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Publication types

Grants and funding

This work was supported by grants from the University of Sharjah to Rizwan Qaisar (Competitive grant # 1901090157, Target grant # 1901090168). We are thankful to Waleed Yousaf, Faisal Nadeem & Ahmad Hassan Nadeem for helping with data analysis.