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Review
. 2021 Oct 5;10(10):2657.
doi: 10.3390/cells10102657.

The Dictyostelium Centrosome

Ralph Gräf  1 , Marianne Grafe  1 , Irene Meyer  1 , Kristina Mitic  1 , Valentin Pitzen  1
Affiliations
Review

The Dictyostelium Centrosome

Ralph Gräf et al. Cells. .

Abstract

The centrosome of Dictyostelium amoebae contains no centrioles and consists of a cylindrical layered core structure surrounded by a corona harboring microtubule-nucleating γ-tubulin complexes. It is the major centrosomal model beyond animals and yeasts. Proteomics, protein interaction studies by BioID and superresolution microscopy methods led to considerable progress in our understanding of the composition, structure and function of this centrosome type. We discuss all currently known components of the Dictyostelium centrosome in comparison to other centrosomes of animals and yeasts.

Keywords: Dictyostelium; centrosome; microtubule-organization; microtubule-organizing center; mitosis.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Schematic comparison of centrosomal structures in animals (A), Dictyostelium (B), and budding yeast (C). Functionally or topologically related structures are drawn in corresponding colors. Taken from [4].
Figure 2
Figure 2
The Dictyostelium centrosome cycle. Nuclei and centrosomes are shown in schematic cross sections, except for the prophase and prometaphase images where a surface view is shown. See text for a detailed description. Redrawn and adapted from [33].
Figure 3
Figure 3
(A) Schematic of subcentrosomal distribution of the major centrosome components. The color code given with the protein names refers to the individual centrosomal substructures; (B) Expansion microscopy of nanobody-labeled Cep192 (magenta) and CP39 (green). Bar = 0.25 µm.
Figure 4
Figure 4
Centrosome-Nucleus-Centromere cluster. (A) Immunoelectron microscopy image showing one section of an isolated nucleus with the attached centrosome. Nuclei were labeled with an antibody against Dictyostelium Sun1 and nanogold conjugated anti-rabbit antibodies. The centrosome (Cn), the centromeric cluster (Cm), the nuclear envelope (NE) and the endoplasmic reticulum (ER) are indicated (image by Prof. Otto Baumann); (B) Immunofluorescence microscopy image of a Sun1-GFP knock-in cell (green) stained with an antibody against the centrosomal core protein CP91 and anti-rabbit-AlexaFluor 568 conjugates (red) and DAPI (blue). The cell edges are outlined by a dashed line. Taken from [243].
See this image and copyright information in PMC

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