Recent progress in optical clearing of eye tissues

doi:10.1016/j.exer.2021.108796

Review

. 2021 Nov:212:108796.

doi: 10.1016/j.exer.2021.108796. Epub 2021 Oct 15.

Recent progress in optical clearing of eye tissues

Wenhan Lu¹, Zhenle Pei², Wei Hu², Chen Tan³, Xiaoyu Tong², Yi Feng⁴, Xinghuai Sun⁵

Affiliations

¹ Department of Ophthalmology & Visual Science, Eye & ENT Hospital, Shanghai Medical College, Fudan University, Shanghai, 200031, China; Department of Integrative Medicine and Neurobiology, State Key Lab of Medical Neurobiology, Institute of Integrative Medicine of Fudan University, Institute of Brain Science, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China.
² Department of Integrative Medicine and Neurobiology, State Key Lab of Medical Neurobiology, Institute of Integrative Medicine of Fudan University, Institute of Brain Science, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China.
³ Department of Ophthalmology & Visual Science, Eye & ENT Hospital, Shanghai Medical College, Fudan University, Shanghai, 200031, China.
⁴ Department of Integrative Medicine and Neurobiology, State Key Lab of Medical Neurobiology, Institute of Integrative Medicine of Fudan University, Institute of Brain Science, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China. Electronic address: fengyi17@fudan.edu.cn.
⁵ Department of Ophthalmology & Visual Science, Eye & ENT Hospital, Shanghai Medical College, Fudan University, Shanghai, 200031, China; State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, Institutes of Brain Science, Fudan University, Shanghai, 200032, China; NHC Key Laboratory of Myopia, Chinese Academy of Medical Sciences, And Shanghai Key Laboratory of Visual Impairment and Restoration (Fudan University), Shanghai, 200031, China. Electronic address: xhsun@shmu.edu.cn.

PMID: 34662543
DOI: 10.1016/j.exer.2021.108796

Free article

Review

Recent progress in optical clearing of eye tissues

Wenhan Lu et al. Exp Eye Res. 2021 Nov.

Free article

. 2021 Nov:212:108796.

doi: 10.1016/j.exer.2021.108796. Epub 2021 Oct 15.

Authors

Wenhan Lu¹, Zhenle Pei², Wei Hu², Chen Tan³, Xiaoyu Tong², Yi Feng⁴, Xinghuai Sun⁵

Affiliations

¹ Department of Ophthalmology & Visual Science, Eye & ENT Hospital, Shanghai Medical College, Fudan University, Shanghai, 200031, China; Department of Integrative Medicine and Neurobiology, State Key Lab of Medical Neurobiology, Institute of Integrative Medicine of Fudan University, Institute of Brain Science, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China.
² Department of Integrative Medicine and Neurobiology, State Key Lab of Medical Neurobiology, Institute of Integrative Medicine of Fudan University, Institute of Brain Science, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China.
³ Department of Ophthalmology & Visual Science, Eye & ENT Hospital, Shanghai Medical College, Fudan University, Shanghai, 200031, China.
⁴ Department of Integrative Medicine and Neurobiology, State Key Lab of Medical Neurobiology, Institute of Integrative Medicine of Fudan University, Institute of Brain Science, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China. Electronic address: fengyi17@fudan.edu.cn.
⁵ Department of Ophthalmology & Visual Science, Eye & ENT Hospital, Shanghai Medical College, Fudan University, Shanghai, 200031, China; State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, Institutes of Brain Science, Fudan University, Shanghai, 200032, China; NHC Key Laboratory of Myopia, Chinese Academy of Medical Sciences, And Shanghai Key Laboratory of Visual Impairment and Restoration (Fudan University), Shanghai, 200031, China. Electronic address: xhsun@shmu.edu.cn.

PMID: 34662543
DOI: 10.1016/j.exer.2021.108796

Erratum in

Corrigendum to 'Recent progress in optical clearing of eye tissues' [Exp. Eye Res. (Nov; 2021) 212 108796].
Lu W, Pei Z, Hu W, Tan C, Tong X, Feng Y, Sun X. Lu W, et al. Exp Eye Res. 2022 Jan;214:108896. doi: 10.1016/j.exer.2021.108896. Epub 2021 Dec 11. Exp Eye Res. 2022. PMID: 34903367 No abstract available.

Abstract

The growing need for viewing the detailed 3D structures of various tissues and organs requires advanced tissue processing and imaging techniques. However, light scattering by tissues hinders detailed structural observations. To overcome this, the emerging technique of "tissue optical clearing" has been flourishing in recent decades, providing excellent opportunities for imaging deep, micro-scale structures of various organs, or even of the whole body. In recent years, advanced tissue clearing techniques have been optimized for specific tissues and organs. Among these tissues, the eye is unique owing to its delicate structure and pigmented retinal epithelial cells, calling for more work on making these tissues "transparent". In this review, we searched Medline and Embase for studies published between January 2006 and August 2021 using the terms "tissue optical clearing", "ophthalmology", "eye", and "optical clearing agents", and we reviewed the publications on the optical clearing techniques of eye tissue from 2006 to the present, including both the clearing procedures and the subsequent analytical processes, thus gaining more insight into the application of tissue optical clearing in basic eye research. Furthermore, we discuss the future potential of optical clearing applications in clinical ophthalmology.

Keywords: Imaging techniques; Ophthalmology; Optical clearing agents; Post-imaging processing; Tissue optical clearing.

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Recent progress in optical clearing of eye tissues

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Recent progress in optical clearing of eye tissues

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