Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1978 Mar;19(3):1076-82.
doi: 10.1128/iai.19.3.1076-1082.1978.

Cellular release of heat-labile enterotoxin of Escherichia coli by bacteriophage induction

P Gemski, A D O'Brien, J A Wohlhieter

Cellular release of heat-labile enterotoxin of Escherichia coli by bacteriophage induction

P Gemski et al. Infect Immun. 1978 Mar.

Abstract

Treatment of some enterotoxigenic Escherichia coli strains with the antibiotic mitomycin C resulted in lysis of the bacteria. Heat-labile enterotoxin (LT) activity of culture filtrates, determined by means of the Y-1 adrenal cell assay, increased dramatically as lysis of the culture proceeded. Further studies with E. coli strains 263 and B21-4 revealed that lysis is due to mitomycin C induction of vetetative development of a temperature bacteriophage. These findings suggest that the elevated levels of LT detected after mitomycin C treatment reflect the lytic release of cell-bound LT rather than the induction by mitomycin C of de novo toxin biosynthesis. Comparable increases in LT activity also resulted from thermal induction of a phage P1Cm lysogen of strain 263 or from sonic disruption of enterotoxigenic strains.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Proc Natl Acad Sci U S A. 1975 Dec;72(12):5041-5 - PubMed
    1. Infect Immun. 1975 Feb;11(2):334-6 - PubMed
    1. Infect Immun. 1975 Dec;12(6):1271-5 - PubMed
    1. Virology. 1972 Jun;48(3):679-89 - PubMed
    1. Infect Immun. 1974 Feb;9(2):342-7 - PubMed

MeSH terms