The Role of miRNAs 340-5p, 92a-3p, and 381-3p in Patients with Endometriosis: A Plasma and Mesenchymal Stem-Like Cell Study
- PMID: 34631883
- PMCID: PMC8494557
- DOI: 10.1155/2021/5298006
The Role of miRNAs 340-5p, 92a-3p, and 381-3p in Patients with Endometriosis: A Plasma and Mesenchymal Stem-Like Cell Study
Abstract
Background: Endometriosis is the most prevalent gynecological disease with elusive etiology. The mysterious entity and the lack of noninvasive diagnostic methods affect women's lives negatively. This study is aimed at finding the relationship between miR-340-5p, 92a-3p, and miR-381-3p and the pathogenesis of endometriosis in endometrial mesenchymal stem-like cells (eMSCs) of endometriosis and assessing their potential as a noninvasive biomarker in plasma.
Methods: Peripheral blood and eMSC specimens were collected from suspected women of endometriosis before laparoscopy. Total RNA was isolated from plasma and cultured eMSCs to synthesize complementary DNA. The expression of miR-340-5p, miR-92a-3p, and miR-381-3p was analyzed by RT-qPCR. To understand these miRNAs' role, we also did a bioinformatic analysis.
Results: There was a downregulation of miR-340-5p, miR-92a-3p, and miR-381-3p in plasma, and the upregulation of miR-340-5p and the downregulation of miR-92a-3p and miR-381-3p in eMSCs of women with endometriosis. There was a positive concordance between the expression of miR-92a-3p and miR-381-3p in plasma and eMSCs. Our study also showed three genes, Solute Carrier Family 6 Member 8 (SLC6A8), Zinc Finger Protein 264 (ZNF264), and mouse double minute 2 (MDM2), as common targets of these miRNAs.
Conclusions: This study has been one of the first attempts to examine the expression of miR-340-5p, miR-92a-3p, and miR-381-3p in both plasma and eMSCs and revealed their possible role in endometriosis based on in silico analysis. Biomarkers pave the way to develop a new therapeutic approach to the management or treatment of endometriosis patients. Our result as a first report shows that combined levels of miRNAs 340-5p and 381-3p may have the potential to be utilized as diagnostic biomarkers for endometriosis.
Copyright © 2021 Afshin Bahramy et al.
Conflict of interest statement
The authors declare no conflict of interest.
Figures
Similar articles
-
A Panel of Plasma miRNAs 199b-3p, 224-5p and Let-7d-3p as Non-Invasive Diagnostic Biomarkers for Endometriosis.Reprod Sci. 2021 Apr;28(4):991-999. doi: 10.1007/s43032-020-00415-z. Epub 2021 Jan 4. Reprod Sci. 2021. PMID: 33398851
-
Deregulation of miR-1245b-5p and miR-92a-3p and their potential target gene, GATA3, in epithelial-mesenchymal transition pathway in breast cancer.Cancer Rep (Hoboken). 2024 Feb;7(2):e1955. doi: 10.1002/cnr2.1955. Epub 2024 Jan 3. Cancer Rep (Hoboken). 2024. PMID: 38173189 Free PMC article.
-
Serum Exosomal MicroRNAs as Potential Circulating Biomarkers for Endometriosis.Dis Markers. 2020 Jan 23;2020:2456340. doi: 10.1155/2020/2456340. eCollection 2020. Dis Markers. 2020. PMID: 32076458 Free PMC article.
-
The role of small extracellular vesicle-miRNAs in endometriosis.Hum Reprod. 2023 Dec 4;38(12):2296-2311. doi: 10.1093/humrep/dead216. Hum Reprod. 2023. PMID: 37877421 Free PMC article. Review.
-
The miRNA Mirage: How Close Are We to Finding a Non-Invasive Diagnostic Biomarker in Endometriosis? A Systematic Review.Int J Mol Sci. 2018 Feb 17;19(2):599. doi: 10.3390/ijms19020599. Int J Mol Sci. 2018. PMID: 29463003 Free PMC article. Review.
Cited by
-
The role of the tumor microenvironment in colorectal cancer and the potential therapeutic approaches.J Clin Lab Anal. 2022 Aug;36(8):e24585. doi: 10.1002/jcla.24585. Epub 2022 Jul 8. J Clin Lab Anal. 2022. PMID: 35808903 Free PMC article. Review.
-
Circulating microRNAs as Non-Invasive Biomarkers in Endometriosis Diagnosis-A Systematic Review.Biomedicines. 2024 Apr 17;12(4):888. doi: 10.3390/biomedicines12040888. Biomedicines. 2024. PMID: 38672242 Free PMC article. Review.
-
Small Non-Coding RNAs as New Biomarkers to Evaluate the Quality of the Embryo in the IVF Process.Biomolecules. 2022 Nov 14;12(11):1687. doi: 10.3390/biom12111687. Biomolecules. 2022. PMID: 36421701 Free PMC article.
-
Specific plasma microRNA profiles could be potential non-invasive biomarkers for biochemical pregnancy loss following embryo transfer.BMC Pregnancy Childbirth. 2024 May 8;24(1):351. doi: 10.1186/s12884-024-06488-x. BMC Pregnancy Childbirth. 2024. PMID: 38720272 Free PMC article.
-
Notch signaling pathway: a comprehensive prognostic and gene expression profile analysis in breast cancer.BMC Cancer. 2022 Dec 7;22(1):1282. doi: 10.1186/s12885-022-10383-z. BMC Cancer. 2022. PMID: 36476410 Free PMC article.
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical
Research Materials