Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Jul;10(2):224-232.
doi: 10.52547/rbmb.10.2.224.

Evaluation of NUF2 and GMNN Expression in Prostate Cancer: Potential Biomarkers for Prostate Cancer Screening

Shaghayegh Pezeshki  1 , Payam Hashemi  2 , Alireza Salimi  3 , Sheida Ebrahimi  4 , Mandana Javanzad  5 , Amir Monfaredan  6
Affiliations

Evaluation of NUF2 and GMNN Expression in Prostate Cancer: Potential Biomarkers for Prostate Cancer Screening

Shaghayegh Pezeshki et al. Rep Biochem Mol Biol. 2021 Jul.

Abstract

Background: Prostate cancer (PC) is one of the most abundant cancers among men, and In Iran, has been responsible for 6% of all deaths from cancer in men. NUF2 and GMNN genes are considered as loci of susceptibility to tumorigenesis in humans. Alterations in expression of these genes have been reported in various malignancies. The aim of our study was to test whether different NUF2 and GMNN expression levels are associated with PC incidence and hence, might be considered as new molecular tools for PC screening.

Methods: Biopsy samples from 40 PC patients and 41 healthy Iranian men were used to determine the relative gene expression. After RNA extraction and cDNA synthesis, samples were analyzed using TaqMan Quantitative Real time PCR. Patients' background information, included smoking habits and family histories of PC, were recorded. Stages and grades of their PC were classified by the TNM tumor, node, metastasis (TMN) staging system based on standard guidelines.

Results: NUF2 expression did not significantly differ between the groups, while GMNN expression was significantly greater in the PC specimens than in the controls.

Conclusion: Regarding the significant role of GMNN in various tumor phenotypes, and its importance in PC progression, the alteration in GMNN expression in PC samples vs. controls indicate that the genetic profiling of this cancer might be considered to personalize therapy for each patient in the future.

Keywords: Family history; Geminin (GMNN); NUF2; Prostate cancer; Tumor staging.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
The number of PC patients and healthy controls in the four age groups.
Fig. 2
Fig. 2
The numbers of controls and PC patients without and with family histories of PC; NA: not applicable.
Fig. 3
Fig. 3
Percentages of prostate cancer patients and healthy controls who smoked; NA: not applicable.
Fig. 4
Fig. 4
Relative expression of NUF2 and GMNN in biopsies from cancerous and healthy prostates.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Torre LA, Siegel RL, Ward EM, Jemal A. Global cancer incidence and mortality rates and trends—an update. Cancer Epidemiol Biomarkers Prev. 2016;25(1):16, 27. - PubMed
    1. Hosseini M-S, Hosseini F, Ahmadi A, Mozafari M, Amjadi I. Antiproliferative Activity of Hypericum perforatum, Achillea millefolium, and Aloe vera in Interaction with the Prostatic Activity of CD82. Rep Biochem Mol Biol. 2019;8(3):260, 268. - PMC - PubMed
    1. Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA, Jemal A. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 2018;68(6):394, 424. - PubMed
    1. Kelly SP, Anderson WF, Rosenberg PS, Cook MB. Past, current, and future incidence rates and burden of metastatic prostate cancer in the United States. Eur Urol Focus. 2018;4(1):121, 127. - PMC - PubMed
    1. Hassanipour S, Fathalipour M, Salehiniya H. The incidence of prostate cancer in Iran:a systematic review and meta-analysis. Prostate Int. 2018;6(2):41–45. - PMC - PubMed

LinkOut - more resources