Membrane filter procedure for enumeration of Candida albicans in natural waters
- PMID: 345966
- PMCID: PMC242818
- DOI: 10.1128/aem.35.2.237-242.1978
Membrane filter procedure for enumeration of Candida albicans in natural waters
Abstract
A membrane filter procedure is described for the enumeration of Candida albicans in natural waters. Several hundred milliliters of sample can be examined by filtration through 1.2-micrometer membranes. Selectivity is achieved by the use of a defined (yeast-nitrogen base plus maltos-) agar medium inclusion of the antimicrobial agents chloramphenicol and cycloheximide, and incubation at 37 degrees C. C. albicans colonies are differentiated primarily through color by use of a bismuth salt indicator system. Average recovery of various strains of C. albicans stressed in seawater at 4 degrees C was 82%, compared with those of spread plate controls on a noninhibitory medium. With river water and raw sewage, 90% of typical C. albicans colonies were confirmed as such in a simplified germ tube test. Atypical colonies verified as C. albicans were infrequent (3%). C. tropicalis and Torulopsis candida were the most common false-positive colonies.
Similar articles
-
Evaluation of Mueller-Hinton-agar as a simple medium for the germ tube production of Candida albicans and Candida dubliniensis.Mycoses. 2008 May;51(3):205-8. doi: 10.1111/j.1439-0507.2007.01469.x. Mycoses. 2008. PMID: 18399901
-
[Presumptive identification of Candida albicans by rapid diagnostic tests].Klin Mikrobiol Infekc Lek. 2006 Oct;12(5):180-3. Klin Mikrobiol Infekc Lek. 2006. PMID: 17080351 Czech.
-
Tobacco agar, a new medium for differentiating Candida dubliniensis from Candida albicans.J Clin Microbiol. 2004 Oct;42(10):4796-8. doi: 10.1128/JCM.42.10.4796-4798.2004. J Clin Microbiol. 2004. PMID: 15472343 Free PMC article.
-
New milk medium for germ tube and chlamydoconidia production by Candida albicans.Mycopathologia. 1993 Aug;123(2):95-8. doi: 10.1007/BF01365086. Mycopathologia. 1993. PMID: 8264773
-
Application of CHROMagar Candida for rapid screening of clinical specimens for Candida albicans, Candida tropicalis, Candida krusei, and Candida (Torulopsis) glabrata.J Clin Microbiol. 1996 Jan;34(1):58-61. doi: 10.1128/jcm.34.1.58-61.1996. J Clin Microbiol. 1996. PMID: 8748273 Free PMC article.
Cited by
-
Fungal contamination of indoor public swimming pools and their dominant physical and chemical properties.J Prev Med Hyg. 2022 Jan 31;62(4):E879-E884. doi: 10.15167/2421-4248/jpmh2021.62.4.1594. eCollection 2021 Dec. J Prev Med Hyg. 2022. PMID: 35603246 Free PMC article.
-
Occurrence, antifungal susceptibility, and virulence factors of opportunistic yeasts isolated from Brazilian beaches.Mem Inst Oswaldo Cruz. 2019 Mar 14;114:e180566. doi: 10.1590/0074-02760180566. Mem Inst Oswaldo Cruz. 2019. PMID: 30892381 Free PMC article.
-
Comparison ofin Situ andin Vitro survival ofCandida albicans in seawater.Microb Ecol. 1977 Dec;4(4):291-302. doi: 10.1007/BF02013273. Microb Ecol. 1977. PMID: 24232221
-
Occurrence ofCandida albicans in fresh gull feces in temperate and subtropical areas.Microb Ecol. 1983 Jul;9(2):171-6. doi: 10.1007/BF02015129. Microb Ecol. 1983. PMID: 24221652
-
Yeasts from marine and estuarine waters with different levels of pollution in the state of rio de janeiro, Brazil.Appl Environ Microbiol. 1981 Jan;41(1):173-8. doi: 10.1128/aem.41.1.173-178.1981. Appl Environ Microbiol. 1981. PMID: 16345683 Free PMC article.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources