Bovine Colostrum Supplementation Improves Bone Metabolism in an Osteoporosis-Induced Animal Model

doi:10.3390/nu13092981

. 2021 Aug 27;13(9):2981.

doi: 10.3390/nu13092981.

Bovine Colostrum Supplementation Improves Bone Metabolism in an Osteoporosis-Induced Animal Model

Eirini K Kydonaki¹, Laura Freitas¹, Bruno M Fonseca¹, Henrique Reguengo¹, Carlos Raposo Simón^{2

3}, Ana R Bastos^{4

5}, Emanuel M Fernandes^{4

5}, Raphaël F Canadas^{4

5}, Joaquim Miguel Oliveira^{4

5}, Vitor M Correlo^{4

5}, Rui L Reis^{4

5}, Maria Vliora⁶, Parakevi Gkiata⁶, Yiannis Koutedakis^{6

7}, Georgia Ntina⁸, Rui Pinto^{9

10}, Andres E Carrillo^{11

12}, Franklim Marques¹, Tânia Amorim¹

Affiliations

¹ UCIBIO/REQUIMTE, Faculty of Pharmacy, University of Porto, 4050-313 Porto, Portugal.
² SALURIS, 28108 Madrid, Spain.
³ Department of Pharmacology, Pharmacognosy and Botany, Complutense University, 28040 Madrid, Spain.
⁴ 3B's Research Group, I3Bs-Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Guimarães, Portugal.
⁵ ICVS/3B's-PT Government Associate Laboratory, 4805-017 Guimarães, Portugal.
⁶ School of Sports and Exercise Sciences, University of Thessaly, 42100 Trikala, Greece.
⁷ Faculty of Education, Health and Wellbeing, University of Wolverhampton, Walsall WS1 3BD, UK.
⁸ BME, Biomechanical Solutions, 43150 Karditsa, Greece.
⁹ iMed.UL, Faculty of Pharmacy, University of Lisbon, 1649-003 Lisbon, Portugal.
¹⁰ Joaquim Chaves Saúde, 1495-068 Miraflores Algés, Portugal.
¹¹ Department of Exercise Science, Chatham University, Pittsburgh, PA 15232, USA.
¹² Move-Cor Inc., Pittsburgh, PA 15017, USA.

PMID: 34578859
PMCID: PMC8471956
DOI: 10.3390/nu13092981

Bovine Colostrum Supplementation Improves Bone Metabolism in an Osteoporosis-Induced Animal Model

Eirini K Kydonaki et al. Nutrients. 2021.

. 2021 Aug 27;13(9):2981.

doi: 10.3390/nu13092981.

Authors

Affiliations

¹ UCIBIO/REQUIMTE, Faculty of Pharmacy, University of Porto, 4050-313 Porto, Portugal.
² SALURIS, 28108 Madrid, Spain.
³ Department of Pharmacology, Pharmacognosy and Botany, Complutense University, 28040 Madrid, Spain.
⁴ 3B's Research Group, I3Bs-Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Guimarães, Portugal.
⁵ ICVS/3B's-PT Government Associate Laboratory, 4805-017 Guimarães, Portugal.
⁶ School of Sports and Exercise Sciences, University of Thessaly, 42100 Trikala, Greece.
⁷ Faculty of Education, Health and Wellbeing, University of Wolverhampton, Walsall WS1 3BD, UK.
⁸ BME, Biomechanical Solutions, 43150 Karditsa, Greece.
⁹ iMed.UL, Faculty of Pharmacy, University of Lisbon, 1649-003 Lisbon, Portugal.
¹⁰ Joaquim Chaves Saúde, 1495-068 Miraflores Algés, Portugal.
¹¹ Department of Exercise Science, Chatham University, Pittsburgh, PA 15232, USA.
¹² Move-Cor Inc., Pittsburgh, PA 15017, USA.

PMID: 34578859
PMCID: PMC8471956
DOI: 10.3390/nu13092981

Abstract

Osteoporosis is characterized by bone loss. The present study aims to investigate the effects of bovine colostrum (BC) on bone metabolism using ovariectomized (OVX) and orchidectomized (ORX) rat models. Twenty-seven-week-old Wistar Han rats were randomly assigned as: (1) placebo control, (2) BC supplementation dose 1 (BC1: 0.5 g/day/OVX, 1 g/day/ORX), (3) BC supplementation dose 2 (BC2: 1 g/day/OVX, 1.5 g/day/ORX) and (4) BC supplementation dose 3 (BC3: 1.5 g/day/OVX, 2 g/day/ORX). Bone microarchitecture, strength, gene expression of VEGFA, FGF2, RANKL, RANK and OPG, and bone resorption/formation markers were assessed after four months of BC supplementation. Compared to the placebo, OVX rats in the BC1 group exhibited significantly higher cortical bone mineral content and trabecular bone mineral content (p < 0.01), while OVX rats in the BC3 group showed significantly higher trabecular bone mineral content (p < 0.05). ORX rats receiving BC dose 2 demonstrated significantly higher levels of trabecular bone mineral content (p < 0.05). Serum osteocalcin in the ORX was pointedly higher in all BC supplementation groups than the placebo (BC1: p < 0.05; BC2, BC3: p < 0.001). Higher doses of BC induced significantly higher relative mRNA expression of OPG, VEGFA, FGF2 and RANKL (p < 0.05). BC supplementation improves bone metabolism of OVX and ORX rats, which might be associated with the activation of the VEGFA, FGF2 and RANKL/RANK/OPG pathways.

Keywords: bone; bovine colostrum; osteoporosis; supplementation.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

**Figure 1**
qPCR analysis for VEGFA, FGF2, RANK, RANKL and OPG. (a) qPCR for VEGFA mRNA; (b) qPCR for FGF2 mRNA; (c) qPCR for RANK mRNA; (d) qPCR for RANKL mRNA; (e) qPCR for OPG mRNA; (f) RANKL/OPG ratio calculated by the relative mRNA values of RANKL and OPG. Data are mean ± SD. Significant differences from the control (placebo supplementation group) are presented by * (p < 0.05).

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Cited by

Pharmacological and Non-Pharmacological Agents versus Bovine Colostrum Supplementation for the Management of Bone Health Using an Osteoporosis-Induced Rat Model.
Kydonaki EK, Freitas L, Reguengo H, Simón CR, Bastos AR, Fernandes EM, Canadas RF, Oliveira JM, Correlo VM, Reis RL, Vliora M, Gkiata P, Koutedakis Y, Ntina G, Pinto R, Carrillo AE, Marques F, Amorim T. Kydonaki EK, et al. Nutrients. 2022 Jul 11;14(14):2837. doi: 10.3390/nu14142837. Nutrients. 2022. PMID: 35889794 Free PMC article.

References

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[1] Peck W.A. Consensus development conference: Diagnosis, prophylaxis, and treatment of osteoporosis. Am. J. Med. 1993;94:646–650. doi: 10.1016/0002-9343(93)90218-e. - DOI - PubMed

[2] Peck W.A. Consensus development conference: Diagnosis, prophylaxis, and treatment of osteoporosis. Am. J. Med. 1993;94:646–650. doi: 10.1016/0002-9343(93)90218-e. - DOI - PubMed

[3] On Osteoporosis N.C.D.P., Prevention D. Osteoporosis prevention, diagnosis, and therapy. JAMA. 2001;285:785–795. doi: 10.1001/jama.285.6.785. - DOI - PubMed

[4] On Osteoporosis N.C.D.P., Prevention D. Osteoporosis prevention, diagnosis, and therapy. JAMA. 2001;285:785–795. doi: 10.1001/jama.285.6.785. - DOI - PubMed

[5] Bliuc D., Nguyen N.D., Alarkawi D., Nguyen T.V., Eisman J.A., Center J.R. Accelerated bone loss and increased post-fracture mortality in elderly women and men. Osteoporos. Int. 2015;26:1331–1339. doi: 10.1007/s00198-014-3014-9. - DOI - PubMed

[6] Bliuc D., Nguyen N.D., Alarkawi D., Nguyen T.V., Eisman J.A., Center J.R. Accelerated bone loss and increased post-fracture mortality in elderly women and men. Osteoporos. Int. 2015;26:1331–1339. doi: 10.1007/s00198-014-3014-9. - DOI - PubMed

[7] Cauley J.A. Public health impact of osteoporosis. J. Gerontol. A Biol. Sci. Med. Sci. 2013;68:1243–1251. doi: 10.1093/gerona/glt093. - DOI - PMC - PubMed

[8] Cauley J.A. Public health impact of osteoporosis. J. Gerontol. A Biol. Sci. Med. Sci. 2013;68:1243–1251. doi: 10.1093/gerona/glt093. - DOI - PMC - PubMed

[9] Adachi J.D., Ioannidis G., Pickard L., Berger C., Prior J.C., Joseph L., Hanley D.A., Olszynski W.P., Murray T.M., Anastassiades T., et al. The association between osteoporotic fractures and health-related quality of life as measured by the Health Utilities Index in the Canadian Multicentre Osteoporosis Study (CaMos) Osteoporos. Int. 2003;14:895–904. doi: 10.1007/s00198-003-1483-3. - DOI - PubMed

[10] Adachi J.D., Ioannidis G., Pickard L., Berger C., Prior J.C., Joseph L., Hanley D.A., Olszynski W.P., Murray T.M., Anastassiades T., et al. The association between osteoporotic fractures and health-related quality of life as measured by the Health Utilities Index in the Canadian Multicentre Osteoporosis Study (CaMos) Osteoporos. Int. 2003;14:895–904. doi: 10.1007/s00198-003-1483-3. - DOI - PubMed

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Bovine Colostrum Supplementation Improves Bone Metabolism in an Osteoporosis-Induced Animal Model

Affiliations

Bovine Colostrum Supplementation Improves Bone Metabolism in an Osteoporosis-Induced Animal Model

Authors

Affiliations

Abstract

Conflict of interest statement

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References

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Grants and funding

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Medical

Abstract

Conflict of interest statement

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References

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Medical