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. 2021 Jun;13(3):312-318.
doi: 10.18502/ijm.v13i3.6393.

Parechovirus and enteroviruses among young infants with sepsis in Iran

Manoochehr Makvandi  1 , Ali Teimoori  1 , Roya Pirmoradi  1 , Chiman Karami  1 , Ahmad Shamsizadeh  2 , Abdolnabi Shabani  1 , Kambiz Ahmadi Angali  3
Affiliations

Parechovirus and enteroviruses among young infants with sepsis in Iran

Manoochehr Makvandi et al. Iran J Microbiol. 2021 Jun.

Abstract

Background and objectives: Human parechoviruses (HPeV) and Human enteroviruses (EV) frequently cause a sepsis-like illness in young infants (younger than three months). Therefore, this study was conducted to determine the frequency of HPeV and EV among the young infants with clinical signs and symptoms of sepsis in Ahvaz city, Iran.

Materials and methods: The blood specimens were collected from 100 (younger than 90 days hospitalized infants) including 54 (56.25%) males and 46 (43.75%) females with clinical signs and symptoms of sepsis-like disease. The RNA was extracted and tested for detection of VP1 region of HPeV and 5 UTR (Untranslated Region) of EV by RT-PCR. The sequences of positive of HPeV were further analyzed to determine HPeV genotyping.

Results: 5/100 (5%) of patients including 2/46 (2%) females and 3/54 (3%) males tested positive for HPeV (P=0.85). The analysis of 5 positive VP1 region of HPeV revealed the genotype 1. The analysis of sequencing and phylogenetic tree revealed that the isolated HPeVs were genotype 1. While 38/100 (38%) specimens including 16 (16%) females and 22 (22%) males were tested positive for EV (P=0.68).

Conclusion: The frequency of HPeV genotype 1 was 5% among the young infants with sepsis. While frequency of EV was 38% among the young infants with sepsis. This study showed HPeV genotype 1 and EV are dominant in this region.

Keywords: Enterovirus; Genotype; Parechovirus; Reverse transcriptase polymerase chain reaction; Sepsis.

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Figures

Fig. 1.
Fig. 1.
A phylogenic tree was constructed with Neighbor joining method for partial VP1 region of two isolated HPeV from two patients with sepsis in Ahvaz city. The two isolated HPeV-1 with accession numbers (MN845069, MN845070) were compared with the different HPeVes genotypes retrieved from GenBank. The results of phylogenetic revealed that the Iranian isolates with black circle color are cluster with HPeV genotype 1 isolated from MK792785.1 (Tehran, Iran), and Belarus (MK167999.1, MK168003.1, MK167997.1, MK167996.1, MK167994.1, MG570229.1). The accuracy was assessed by 1000 bootstrap replicates. Scale bar=0.05
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References

    1. Brent AJ. Sepsis. Medicine 2018;45:649–653.
    1. Dolin HH, Papadimos TJ, Chen X, Pan ZK. Characterization of pathogenic sepsis etiologies and patient profiles: a novel approach to triage and treatment. Microbiol Insights 2019;12:1178636118825081. - PMC - PubMed
    1. Bone RC. The sepsis syndrome. definition and general approach to management. Clin Chest Med 1996;17:175–181. - PubMed
    1. Gupta N, Richter R, Robert S, Kong M. Viral sepsis in children. Front Pediatr 2018;6:252. - PMC - PubMed
    1. Tapparel C, Siegrist F, Petty TJ, Kaiser L. Picornavirus and enterovirus diversity with associated human diseases. Infect Genet Evol 2013;14:282–293. - PubMed

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