Single nucleus RNA-sequencing: how it's done, applications and limitations
- PMID: 34515767
- DOI: 10.1042/ETLS20210074
Single nucleus RNA-sequencing: how it's done, applications and limitations
Abstract
Single nuclei RNA-sequencing (sNuc-Seq) is a methodology which uses isolated nuclei instead of whole cells to profile gene expression. By using droplet microfluidic technologies, users are able to profile thousands of single transcriptomes at high throughput from their chosen tissue. This article aims to introduce sNuc-Seq as a method and its utility in multiple tissue types. Furthermore, we discuss the risks associated with the use of nuclei, which must be considered before committing to a methodology.
Keywords: DroNc-Seq; NGS; sNuc-Seq; scRNA-Seq; single cell.
© 2021 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society and the Royal Society of Biology.
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