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. 2021 Aug 11;12(1):451.
doi: 10.1186/s13287-021-02500-9.

MSCs from polytrauma patients: preliminary comparative study with MSCs from elective-surgery patients

Raúl López #  1 , Gerardo J Martí-Chillón #  2 , Juan F Blanco  3   4   5   6 , Carmen da Casa  7 , Javier González-Robledo  8 , David Pescador  1   7 , Silvia Preciado  2   9 , Sandra Muntión  2   10   9 , Fermín Sánchez-Guijo  2   11   10   9
Affiliations

MSCs from polytrauma patients: preliminary comparative study with MSCs from elective-surgery patients

Raúl López et al. Stem Cell Res Ther. .

Abstract

Background: Polytrauma is a major clinical problem due to its impact on morbidity and mortality, especially among the younger population. Its pathophysiology is not completely elucidated, and the study of the involvement of certain cell populations with therapeutic potential, such as mesenchymal stromal cells (MSCs), is an area of growing interest, as mesenchymal cells have anti-inflammatory, immunoregulatory, and osteogenic potential.

Methods: In the present preliminary work, we have evaluated the characteristics of MSCs in terms of proliferation, immunophenotype, cell cycle, clonogenic capacity, and multilineage differentiation ability in a series of 18 patients with polytrauma and compared them to those from otherwise healthy patients undergoing elective spinal surgery.

Results: MSCs from polytrauma patients displayed higher proliferative potential with significantly higher cumulative population doublings, increased expression of some important cell adhesion molecules (CD105, CD166), and an early pre-osteogenic differentiation ability compared to those of the control group.

Conclusions: MSCs could potentially be of help in the repair process of polytrauma patients contribute to both cell-tissue repair and anti-inflammatory response. This potential should be further explored in larger studies.

Keywords: Bone regeneration; Cellular therapy; MSC; Mesenchymal stem cells; Mesenchymal stromal cells; Polytrauma.

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Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
Mean fluorescence intensity (MFI) differential expression of positive MSC membrane markers. Expression of CD44, CD105, CD90, CD73, and CD166 in polytrauma MSCs (gray dots) and control MSCs (black triangles). Bars are expressing median and interquartile range. “*” indicates p < 0.05 on group comparisons
Fig. 2
Fig. 2
Mean cumulative population doubling (CPD) of control MSCs (blue) and polytraumatized-derived MSCs (red). Error bars are representing standard deviation. “*” indicates p < 0.05 on group comparisons at each stage
Fig. 3
Fig. 3
In vitro MSC multilineage differentiation. Control group (A–E) and polytrauma group (F–J). Oil-Red staining (10×) after 21 days of culture (A/F). Alkaline phosphatase staining (10×) after MSC culture with baseline expansion medium (B/G) and osteodiff medium (C/H) for 10 days. Alizarin-Red staining (10×) with baseline expansion medium (D/I) and osteodiff medium (E/J) for 21 days. Scale bar: 100 μm
Fig. 4
Fig. 4
Fold increase in mRNA levels of the cited genes. For adipocyte-differentiated MSCs for 21 days (A), chondrocyte-differentiated MSCs for 21 days (B), and osteoblast-differentiated MSCs for 10 days (C) and 21 days (D)
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