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. 2021 Nov;53(10):e14198.
doi: 10.1111/and.14198. Epub 2021 Aug 10.

Xuefu Zhuyu decoction improves asthma-induced asthenozoospermia based on network pharmacology and in vivo experiment

Sheng Deng  1   2 , Xuefeng Gong  1   3 , Zhongwen Long  1   2 , Binghao Bao  1   2 , Fanchao Meng  1   2 , Junlong Feng  1   2 , Hao Kuang  1   2 , Haisong Li  2 , Bin Wang  2 , Jisheng Wang  1   2   4
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Xuefu Zhuyu decoction improves asthma-induced asthenozoospermia based on network pharmacology and in vivo experiment

Sheng Deng et al. Andrologia. 2021 Nov.

Abstract

This study aimed to verify that Xuefu Zhuyu decoction (XFZYD) can improve asthenozoospermia caused by asthma, and explore its potential mechanism. Ovalbumin solution is used to induce asthma rat models. Sperm concentration and motility are used to evaluate semen quality. Immunohistochemistry (IHC), Western blotting and real-time reverse transcription-quantitative polymerase chain reaction (RT-qPCR) are used to detect proteins and mRNA related to rat testis tissue. Haematoxylin and eosin (H&E) staining was used to observe changes in testicular tissues. Through network pharmacology, eriodictyol, 18-β-glycyrrhetinic acid, naringenin, chrysin and Hispidulin were prominent active ingredients of XFZYD. We found that XFZYD regulates the expression levels of albumin (ALB), vascular endothelial growth factor A (VEGFA), interleukin 6 (IL-6) protein and mRNA, thereby improving the histopathological morphology of the testis, increasing the concentration and motility of spermatozoa. We suggest that future research can increase the detection of hormones and oxidative stress and other related indicators, so as to conduct more in-depth exploration.

Keywords: Xuefu Zhuyu Decoction; asthenozoospermia; asthmatic attack; network pharmacology.

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