Tissue clearing to examine tumour complexity in three dimensions

doi:10.1038/s41568-021-00382-w

Review

. 2021 Nov;21(11):718-730.

doi: 10.1038/s41568-021-00382-w. Epub 2021 Jul 30.

Tissue clearing to examine tumour complexity in three dimensions

Jorge Almagro^#¹, Hendrik A Messal^#², May Zaw Thin³, Jacco van Rheenen², Axel Behrens^{4

5

6}

Affiliations

¹ Adult Stem Cell Laboratory, The Francis Crick Institute, London, UK.
² Department of Molecular Pathology, Oncode Institute, Netherlands Cancer Institute, Amsterdam, The Netherlands.
³ Cancer Stem Cell Laboratory, Institute of Cancer Research, London, UK.
⁴ Adult Stem Cell Laboratory, The Francis Crick Institute, London, UK. axel.behrens@icr.ac.uk.
⁵ Cancer Stem Cell Laboratory, Institute of Cancer Research, London, UK. axel.behrens@icr.ac.uk.
⁶ Convergence Science Centre and Division of Cancer, Department of Surgery and Cancer, Imperial College London, London, UK. axel.behrens@icr.ac.uk.

^# Contributed equally.

PMID: 34331034
DOI: 10.1038/s41568-021-00382-w

Review

Tissue clearing to examine tumour complexity in three dimensions

Jorge Almagro et al. Nat Rev Cancer. 2021 Nov.

. 2021 Nov;21(11):718-730.

doi: 10.1038/s41568-021-00382-w. Epub 2021 Jul 30.

Authors

Jorge Almagro^#¹, Hendrik A Messal^#², May Zaw Thin³, Jacco van Rheenen², Axel Behrens^{4

5

6}

Affiliations

¹ Adult Stem Cell Laboratory, The Francis Crick Institute, London, UK.
² Department of Molecular Pathology, Oncode Institute, Netherlands Cancer Institute, Amsterdam, The Netherlands.
³ Cancer Stem Cell Laboratory, Institute of Cancer Research, London, UK.
⁴ Adult Stem Cell Laboratory, The Francis Crick Institute, London, UK. axel.behrens@icr.ac.uk.
⁵ Cancer Stem Cell Laboratory, Institute of Cancer Research, London, UK. axel.behrens@icr.ac.uk.
⁶ Convergence Science Centre and Division of Cancer, Department of Surgery and Cancer, Imperial College London, London, UK. axel.behrens@icr.ac.uk.

^# Contributed equally.

PMID: 34331034
DOI: 10.1038/s41568-021-00382-w

Abstract

The visualization of whole organs and organisms through tissue clearing and fluorescence volumetric imaging has revolutionized the way we look at biological samples. Its application to solid tumours is changing our perception of tumour architecture, revealing signalling networks and cell interactions critical in tumour progression, and provides a powerful new strategy for cancer diagnostics. This Review introduces the latest advances in tissue clearing and three-dimensional imaging, examines the challenges in clearing epithelia - the tissue of origin of most malignancies - and discusses the insights that tissue clearing has brought to cancer research, as well as the prospective applications to experimental and clinical oncology.

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References

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1. Ueda, H. R. et al. Tissue clearing and its applications in neuroscience. Nat. Rev. Neurosci. 21, 61–79 (2020). - PubMed - PMC - DOI
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[1] Spalteholz, W. Über das Durchsichtigmachen von menschlichen und tierischen Präparaten und seine theoretischen Bedingungen [German] (S. Hirzel, 1914).

[2] Spalteholz, W. Über das Durchsichtigmachen von menschlichen und tierischen Präparaten und seine theoretischen Bedingungen [German] (S. Hirzel, 1914).

[3] Huisken, J., Swoger, J., Del Bene, F., Wittbrodt, J. & Stelzer, E. H. Optical sectioning deep inside live embryos by selective plane illumination microscopy. Science 305, 1007–1009 (2004). - PubMed - DOI

[4] Huisken, J., Swoger, J., Del Bene, F., Wittbrodt, J. & Stelzer, E. H. Optical sectioning deep inside live embryos by selective plane illumination microscopy. Science 305, 1007–1009 (2004). - PubMed - DOI

[5] Voie, A. H., Burns, D. H. & Spelman, F. A. Orthogonal-plane fluorescence optical sectioning: three-dimensional imaging of macroscopic biological specimens. J. Microsc. 170, 229–236 (1993). - PubMed - DOI

[6] Voie, A. H., Burns, D. H. & Spelman, F. A. Orthogonal-plane fluorescence optical sectioning: three-dimensional imaging of macroscopic biological specimens. J. Microsc. 170, 229–236 (1993). - PubMed - DOI

[7] Ueda, H. R. et al. Tissue clearing and its applications in neuroscience. Nat. Rev. Neurosci. 21, 61–79 (2020). - PubMed - PMC - DOI

[8] Ueda, H. R. et al. Tissue clearing and its applications in neuroscience. Nat. Rev. Neurosci. 21, 61–79 (2020). - PubMed - PMC - DOI

[9] Tedeschi, A. et al. Cep55 promotes cytokinesis of neural progenitors but is dispensable for most mammalian cell divisions. Nat. Commun. 11, 1746 (2020). - PubMed - PMC - DOI

[10] Tedeschi, A. et al. Cep55 promotes cytokinesis of neural progenitors but is dispensable for most mammalian cell divisions. Nat. Commun. 11, 1746 (2020). - PubMed - PMC - DOI

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Tissue clearing to examine tumour complexity in three dimensions

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Tissue clearing to examine tumour complexity in three dimensions

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