Immunophenotypic characterization of double positive T lymphocytes in Cuban older adults

doi:10.1016/j.exger.2021.111450

. 2021 Sep:152:111450.

doi: 10.1016/j.exger.2021.111450. Epub 2021 Jun 10.

Immunophenotypic characterization of double positive T lymphocytes in Cuban older adults

Yenisey Triana Marrero¹, Vianed Marsán Suárez², Consuelo Milagros Macías Abraham³, Imilla Casado Hernández⁴, Elizabeth Hernández Ramos⁵, Gabriela Díaz Domínguez⁶, Yaneisy Duarte Pérez⁷, Mary Carmen Reyes Zamora⁸, Luis Felipe Heredia Guerra⁹

Affiliations

¹ Department of Immunology, "Dr. José Manuel BallesterSantovenia" Institute of Hematology and Immunology, Calle 19 e/8 y 10., Postal mail 8070, CP 10800, Vedado, Havana, Cuba. Electronic address: yeniseyt@infomed.sld.cu.
² Department of Immunology, "Dr. José Manuel BallesterSantovenia" Institute of Hematology and Immunology, Calle 19 e/8 y 10., Postal mail 8070, CP 10800, Vedado, Havana, Cuba. Electronic address: vmarsan@infomed.sld.cu.
³ Department of Immunology, "Dr. José Manuel BallesterSantovenia" Institute of Hematology and Immunology, Calle 19 e/8 y 10., Postal mail 8070, CP 10800, Vedado, Havana, Cuba. Electronic address: cmabraham@infomed.sld.cu.
⁴ Department of Immunology, "Dr. José Manuel BallesterSantovenia" Institute of Hematology and Immunology, Calle 19 e/8 y 10., Postal mail 8070, CP 10800, Vedado, Havana, Cuba. Electronic address: icasado@infomed.sld.cu.
⁵ Department of Immunology, "Dr. José Manuel BallesterSantovenia" Institute of Hematology and Immunology, Calle 19 e/8 y 10., Postal mail 8070, CP 10800, Vedado, Havana, Cuba. Electronic address: ramos.beth94@gmail.com.
⁶ Department of Immunology, "Dr. José Manuel BallesterSantovenia" Institute of Hematology and Immunology, Calle 19 e/8 y 10., Postal mail 8070, CP 10800, Vedado, Havana, Cuba. Electronic address: gdiazdominguez@infomed.sld.cu.
⁷ Department of Immunology, "Dr. José Manuel BallesterSantovenia" Institute of Hematology and Immunology, Calle 19 e/8 y 10., Postal mail 8070, CP 10800, Vedado, Havana, Cuba. Electronic address: dpyaneisy@gmail.com.
⁸ National Center for Biopreparations, Bejucal, Mayabeque, Cuba. Electronic address: mcarmen@reyesbiocen.cu.
⁹ Subdirection for Medical Assistance, Longevity, Anging and Health Research Center (CITED), Vedado, Havana, Cuba. Electronic address: viceamcited@infomed.sld.cu.

PMID: 34119608
DOI: 10.1016/j.exger.2021.111450

Immunophenotypic characterization of double positive T lymphocytes in Cuban older adults

Yenisey Triana Marrero et al. Exp Gerontol. 2021 Sep.

. 2021 Sep:152:111450.

doi: 10.1016/j.exger.2021.111450. Epub 2021 Jun 10.

Authors

Affiliations

¹ Department of Immunology, "Dr. José Manuel BallesterSantovenia" Institute of Hematology and Immunology, Calle 19 e/8 y 10., Postal mail 8070, CP 10800, Vedado, Havana, Cuba. Electronic address: yeniseyt@infomed.sld.cu.
² Department of Immunology, "Dr. José Manuel BallesterSantovenia" Institute of Hematology and Immunology, Calle 19 e/8 y 10., Postal mail 8070, CP 10800, Vedado, Havana, Cuba. Electronic address: vmarsan@infomed.sld.cu.
³ Department of Immunology, "Dr. José Manuel BallesterSantovenia" Institute of Hematology and Immunology, Calle 19 e/8 y 10., Postal mail 8070, CP 10800, Vedado, Havana, Cuba. Electronic address: cmabraham@infomed.sld.cu.
⁴ Department of Immunology, "Dr. José Manuel BallesterSantovenia" Institute of Hematology and Immunology, Calle 19 e/8 y 10., Postal mail 8070, CP 10800, Vedado, Havana, Cuba. Electronic address: icasado@infomed.sld.cu.
⁵ Department of Immunology, "Dr. José Manuel BallesterSantovenia" Institute of Hematology and Immunology, Calle 19 e/8 y 10., Postal mail 8070, CP 10800, Vedado, Havana, Cuba. Electronic address: ramos.beth94@gmail.com.
⁶ Department of Immunology, "Dr. José Manuel BallesterSantovenia" Institute of Hematology and Immunology, Calle 19 e/8 y 10., Postal mail 8070, CP 10800, Vedado, Havana, Cuba. Electronic address: gdiazdominguez@infomed.sld.cu.
⁷ Department of Immunology, "Dr. José Manuel BallesterSantovenia" Institute of Hematology and Immunology, Calle 19 e/8 y 10., Postal mail 8070, CP 10800, Vedado, Havana, Cuba. Electronic address: dpyaneisy@gmail.com.
⁸ National Center for Biopreparations, Bejucal, Mayabeque, Cuba. Electronic address: mcarmen@reyesbiocen.cu.
⁹ Subdirection for Medical Assistance, Longevity, Anging and Health Research Center (CITED), Vedado, Havana, Cuba. Electronic address: viceamcited@infomed.sld.cu.

PMID: 34119608
DOI: 10.1016/j.exger.2021.111450

Abstract

Introduction: Immunosenescence is the functional deterioration of the immune system during natural aging that mainly affects T lymphocytes, within which is the subpopulation of peripheral double positive T lymphocytes that are immunomodulators of the immune response. They are increased in older adults and play a fundamental role in the immunopathogenesis of autoimmune diseases, viral infections and neoplasms.

Objective: To characterize the immunophenotype of peripheral double positive T lymphocytes in Cuban older adults.

Methods: A cross-sectional study was carried out in 30 older adults residing in Cuba. Peripheral blood double positive T lymphocytes were quantified by flow cytometry. The reading was done on a Beckman Coulter Gallios flow cytometer. A Beckman Coulter Gallios flow cytometer was used for data analysis. Percentage values, median and standard deviation were used.

Results: There was a predominance of women who represented 70%. No older adults with low values of double positive T lymphocytes were reported. Women with high percentage and absolute values of DPT lymphocytes predominated in relation to men. Among the high percentage values and the absolute value of DPT lymphocytes, the group ≥80 years prevailed.

Conclusions: Most of the older adults who exhibited high percentage and absolute values of DPT lymphocytes had some associated comorbidity, an idea that suggests that CD4 + CD8+ T cells seem to play an important role in peripheral sites as powerful immunosuppressants or as cells with high cytotoxic potential.

Keywords: Diseases; Double positive T lymphocytes; Elderly; Flow cytometry; Immunosenescence.

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Immunophenotypic characterization of double positive T lymphocytes in Cuban older adults

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Immunophenotypic characterization of double positive T lymphocytes in Cuban older adults

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