Analysis of Myc Chromatin Binding by Calibrated ChIP-Seq Approach
- PMID: 34019290
- DOI: 10.1007/978-1-0716-1476-1_8
Analysis of Myc Chromatin Binding by Calibrated ChIP-Seq Approach
Abstract
Here, we present a strategy to map and quantify the interactions between Myc and chromatin using a calibrated Myc ChIP-seq approach. We recommend the use of an internal spike-in control for post-sequencing normalization to enable detection of broad changes in Myc binding as can occur under conditions with varied Myc abundance. We also highlight a range of bioinformatic analyses that can dissect the downstream effects of Myc binding. These methods include peak calling, mapping Myc onto an integrated metagenome, juxtaposing ChIP-seq data with matching RNA-seq data, and identifying gene ontologies enriched for genes with high Myc binding. Our aim is to provide a guided strategy, from cell harvest through to bioinformatic analysis, to elucidate the global effects of Myc on transcription.
Keywords: ChIP-seq; Myc; Spike-in normalization.
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