Holotomography: Refractive Index as an Intrinsic Imaging Contrast for 3-D Label-Free Live Cell Imaging

doi:10.1007/978-981-33-6064-8_10

. 2021:1310:211-238.

doi: 10.1007/978-981-33-6064-8_10.

Holotomography: Refractive Index as an Intrinsic Imaging Contrast for 3-D Label-Free Live Cell Imaging

Doyeon Kim¹, Sangyun Lee², Moosung Lee², Juntaek Oh², Su-A Yang³, YongKeun Park^{4

5

6}

Affiliations

¹ Department of Chemistry, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, South Korea.
² Department of Physics, KAIST, Daejeon, South Korea.
³ Department of Biological Sciences, KAIST, Daejeon, South Korea.
⁴ Department of Physics, KAIST, Daejeon, South Korea. yk.park@kaist.ac.kr.
⁵ KAIST Institute Health Science and Technology, Daejeon, South Korea. yk.park@kaist.ac.kr.
⁶ Tomocube Inc., Daejeon, South Korea. yk.park@kaist.ac.kr.

PMID: 33834439
DOI: 10.1007/978-981-33-6064-8_10

Holotomography: Refractive Index as an Intrinsic Imaging Contrast for 3-D Label-Free Live Cell Imaging

Doyeon Kim et al. Adv Exp Med Biol. 2021.

. 2021:1310:211-238.

doi: 10.1007/978-981-33-6064-8_10.

Authors

Doyeon Kim¹, Sangyun Lee², Moosung Lee², Juntaek Oh², Su-A Yang³, YongKeun Park^{4

5

6}

Affiliations

¹ Department of Chemistry, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, South Korea.
² Department of Physics, KAIST, Daejeon, South Korea.
³ Department of Biological Sciences, KAIST, Daejeon, South Korea.
⁴ Department of Physics, KAIST, Daejeon, South Korea. yk.park@kaist.ac.kr.
⁵ KAIST Institute Health Science and Technology, Daejeon, South Korea. yk.park@kaist.ac.kr.
⁶ Tomocube Inc., Daejeon, South Korea. yk.park@kaist.ac.kr.

PMID: 33834439
DOI: 10.1007/978-981-33-6064-8_10

Abstract

Live cell imaging provides essential information in the investigation of cell biology and related pathophysiology. Refractive index (RI) can serve as intrinsic optical imaging contrast for 3-D label-free and quantitative live cell imaging, and provide invaluable information to understand various dynamics of cells and tissues for the study of numerous fields. Recently significant advances have been made in imaging methods and analysis approaches utilizing RI, which are now being transferred to biological and medical research fields, providing novel approaches to investigate the pathophysiology of cells. To provide insight into how RI can be used as an imaging contrast for imaging of biological specimens, here we provide the basic principle of RI-based imaging techniques and summarize recent progress on applications, ranging from microbiology, hematology, infectious diseases, hematology, and histopathology.

Keywords: Biological imaging; Holotomography; Live cell imaging; Microscopy; Quantitative phase imaging; Refractive index.

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References

1. Allen R, David G, Nomarski G (1969) The zeiss-Nomarski differential interference equipment for transmitted-light microscopy. Z Wiss Mikrosk 69:193–221 - PubMed
1. Barer R (1952) Interference microscopy and mass determination. Nature 169:366–367 - PubMed - DOI
1. Barer R (1953) Determination of dry mass, thickness, solid and water concentration in living cells. Nature 172:1097–1098 - PubMed - DOI
1. Barty A, Nugent K, Roberts A, Paganin D (2000) Quantitative phase tomography. Opt Commun 175:329–336 - DOI
1. Bennet M, Gur D, Yoon J, Park Y, Faivre D (2016) A bacteria-based remotely tunable photonic device. Advanced Optical Materials

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[1] Allen R, David G, Nomarski G (1969) The zeiss-Nomarski differential interference equipment for transmitted-light microscopy. Z Wiss Mikrosk 69:193–221 - PubMed

[2] Allen R, David G, Nomarski G (1969) The zeiss-Nomarski differential interference equipment for transmitted-light microscopy. Z Wiss Mikrosk 69:193–221 - PubMed

[3] Barer R (1952) Interference microscopy and mass determination. Nature 169:366–367 - PubMed - DOI

[4] Barer R (1952) Interference microscopy and mass determination. Nature 169:366–367 - PubMed - DOI

[5] Barer R (1953) Determination of dry mass, thickness, solid and water concentration in living cells. Nature 172:1097–1098 - PubMed - DOI

[6] Barer R (1953) Determination of dry mass, thickness, solid and water concentration in living cells. Nature 172:1097–1098 - PubMed - DOI

[7] Barty A, Nugent K, Roberts A, Paganin D (2000) Quantitative phase tomography. Opt Commun 175:329–336 - DOI

[8] Barty A, Nugent K, Roberts A, Paganin D (2000) Quantitative phase tomography. Opt Commun 175:329–336 - DOI

[9] Bennet M, Gur D, Yoon J, Park Y, Faivre D (2016) A bacteria-based remotely tunable photonic device. Advanced Optical Materials

[10] Bennet M, Gur D, Yoon J, Park Y, Faivre D (2016) A bacteria-based remotely tunable photonic device. Advanced Optical Materials

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Holotomography: Refractive Index as an Intrinsic Imaging Contrast for 3-D Label-Free Live Cell Imaging

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Holotomography: Refractive Index as an Intrinsic Imaging Contrast for 3-D Label-Free Live Cell Imaging

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