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. 2021 Apr 1;18(1):14.
doi: 10.1186/s12950-021-00275-7.

Asthmatic condition induced the activity of exosome secretory pathway in rat pulmonary tissues

Asheed Almohammai  1 , Reza Rahbarghazi  2   3 , Rana Keyhanmanesh  4   5   6 , Jafar Rezaie #  7 , Mahdi Ahmadi #  8   9   10
Affiliations

Asthmatic condition induced the activity of exosome secretory pathway in rat pulmonary tissues

Asheed Almohammai et al. J Inflamm (Lond). .

Abstract

Background: The recent studies highlighted the critical role of exosomes in the regulation of inflammation. Here, we investigated the dynamic biogenesis of the exosomes in the rat model of asthma.

Results: Our finding showed an increase in the expression of IL-4 and the suppression of IL-10 in asthmatic lung tissues compared to the control samples (p < 0.05). Along with the promotion of IL-4, the protein level of TNF-α was induced, showing an active inflammatory status in OVA-sensitized rats. According to our data, the promotion of asthmatic responses increased exosome biogenesis indicated by increased CD63 levels and acetylcholine esterase activity compared to the normal condition (p < 0.05).

Conclusion: Data suggest that the stimulation of inflammatory response in asthmatic rats could simultaneously increase the paracrine activity of pulmonary cells via the exosome biogenesis. Exosome biogenesis may correlate with the inflammatory response.

Keywords: Asthma; CD63; Exosome biogenesis; Inflammatory cytokines; Rats.

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Conflict of interest statement

The authors have no competing interests to declare.

Figures

Fig. 1
Fig. 1
Histopathological examination of asthmatic pulmonary tissue using H&E staining. Asthmatic changes were indicated by prominent interstitial pneumonia (arrows), focal hemorrhagia (arrows heads), and emphysema. The pathological remodeling coincided with the peribronchial cuffing, bronchial smooth muscle hypertrophy, and BALT hyperplasia. These features highlight the efficiency of our protocol in the induction of asthmatic changes using OVA.
Fig. 2
Fig. 2
Measuring the transcription of IL-4 a and IL-10 b mRNA in the lung tissues of control and asthmatic group (n = 8). Bars represent the mean ± SEM. Statistical differences between control and asthmatic group: ++; p < 0.01 and +++; p < 0.001
Fig. 3.
Fig. 3.
TNF-α in BALF of control and asthmatic rats (n = 8). Bars represent the mean ± SEM. Statistical differences between control and asthmatic groups: +++; p < 0.001
Fig. 4
Fig. 4
Western blotting analysis of CD63 protein in lung tissues a. The relative expression of CD63 protein increased in asthmatic lung tissues b. Bars represent the mean ± SEM. Statistical differences between control and asthmatic group: ++; p < 0.01
Fig. 5
Fig. 5
Quantification of the number of exosomes secreted into BAL fluid by acetylcholinesterase (AChE) activity assay. Bars represent the mean ± SEM. Statistical differences between control and asthmatic group: +++; p < 0.001
Fig. 6
Fig. 6
Transmission electron micrographs of isolated exosomes a. Analyzing the size of exosomes diameter purified from BAL samples of asthmatic and control groups b. Flow cytometry confirmed the expression of CD63 marker on exosomes c. Bars represent the mean ± SEM. Statistical differences between control and asthmatic group: +++; p < 0.001
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References

    1. Ahmadi M, Rahbarghazi R, Aslani MR, Shahbazfar AA, Kazemi M, Keyhanmanesh R. Bone marrow mesenchymal stem cells and their conditioned media could potentially ameliorate ovalbumin-induced asthmatic changes. Biomed Pharmacother. 2017;85:28–40. doi: 10.1016/j.biopha.2016.11.127. - DOI - PubMed
    1. To T. Stanojevic S, Moores G, Gershon AS, Bateman ED, Cruz AA, Boulet L-P. Global asthma prevalence in adults: findings from the cross-sectional world health survey. BMC Public Health. 2012;12:204. doi: 10.1186/1471-2458-12-204. - DOI - PMC - PubMed
    1. Keyhanmanesh R, Rahbarghazi R, Aslani MR, Hassanpour M, Ahmadi M. Systemic delivery of mesenchymal stem cells condition media in repeated doses acts as magic bullets in restoring IFN-γ/IL-4 balance in asthmatic rats. Life Sci. 2018;212:30–36. doi: 10.1016/j.lfs.2018.09.049. - DOI - PubMed
    1. Ahmadi M, Rahbarghazi R, Soltani S, Aslani MR, Keyhanmanesh R. Contributory anti-inflammatory effects of mesenchymal stem cells, not conditioned media, on ovalbumin-induced asthmatic changes in male rats. Inflammation. 2016;39:1960–1971. doi: 10.1007/s10753-016-0431-2. - DOI - PubMed
    1. Cañas JA, Sastre B, Rodrigo-Muñoz JM, del Pozo V. Exosomes: a new approach to asthma pathology. Clin Chim Acta. 2019;495:139–147. doi: 10.1016/j.cca.2019.04.055. - DOI - PubMed

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