Genetic polymorphism of ganglioside expression in mouse organs
- PMID: 3360761
- DOI: 10.1093/oxfordjournals.jbchem.a122232
Genetic polymorphism of ganglioside expression in mouse organs
Abstract
In previous studies it was demonstrated that there are three variations as to the expression of liver gangliosides in inbred strains of mice; the first group expresses GM3(NeuGc) as a major component, the second group, GM2(NeuGc), and the third group, GM2(NeuGc), GM1 (NeuGc), and GD1a(NeuGc). In the present study, we attempted to determine which organs, if any, exhibit the same polymorphic variations as those observed in the liver. Thus, the gangliosides in spleen, thymus, heart, lung, kidney, testis, and erythrocytes, as well as those in liver, were examined using a TLC-mapping technique or by one-dimensional TLC. WHT/Ht, BALB/c, and ICR mice, which are typical strains as to the polymorphic expression of liver gangliosides, were used for the analysis. The presence of GM1 was confirmed by not only chemical detection on TLC plates but also with a TLC-immunostaining procedure using choleragenoid. These comparative studies indicated that only erythrocytes exhibited the same polymorphic variations of ganglioside expression as those in the liver, but the other six organs showed specific patterns which were not polymorphic. In addition to this, there were the following two interesting findings. Firstly, WHT/Ht mice, in which GM2(NeuGc) and GM1(NeuGc) are not expressed in the liver and erythrocytes, did not express a detectable amount of GM2(NeuGc) but expressed GM1(NeuGc) in all the other organs. Secondly, marked polymorphic variation was found in the expression of GM4(NeuAc) in the erythrocytes.
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