Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Jan;50(1):69-86.
doi: 10.1007/s00249-020-01491-4. Epub 2021 Jan 13.

Differential dielectroscopic data on the relation of erythrocyte membrane skeleton to erythrocyte deformability and flicker

Ivan T Ivanov  1 , Boyana K Paarvanova  2
Affiliations

Differential dielectroscopic data on the relation of erythrocyte membrane skeleton to erythrocyte deformability and flicker

Ivan T Ivanov et al. Eur Biophys J. 2021 Jan.

Abstract

Two dielectric relaxations, βsp (1.5 MHz) and γ1sp (7 MHz), have been detected on spectrin-based membrane skeleton (MS) of red blood cells (RBCs) using the plot of admittance changes at the spectrin denaturation temperature (Ivanov and Paarvanova in Bioelectrochemistry 110: 59-68, 2016, Electrochim Acta 317: 289-300, 2019a). In this study, we treated RBCs and RBC ghost membranes with agents that make membranes rigid and suppress membrane flicker, and studied the effect on βsp and γ1sp relaxations. Diamide (diazene dicarboxylic acid bis-(N,N-dimethylamide)) (up to 0.85 mM), taurine mustard (tris(2-chloroethyl)amine) (up to 2 mM), known to specifically cross-link and stiffen spectrin, and glutaraldehyde (up to 0.044%) all inhibited the relaxations in RBC ghost membranes. Similar inhibition was obtained resealing RBC ghost membranes with 2,3-diphosphoglicerate (up to 15 mM), binding WGA (wheat germ agglutinin) (up to 0.025 mg/ml) to exofacial aspect of RBCs, incubating RBCs in hypotonic (200 mOsm) and hypertonic (600-900 mOsm) media and depleting RBCs of ATP. By contrast, concanavalin A (1 mg/ml) and DIDS (4,4'-diiso-thiocyanato stilbene-2,2'-disulfonic acid) (75 μM, pH 8.2), both known to bind specifically band 3 integral protein of RBCs without effect on RBC membrane rigidity, did not affect the relaxations. We conclude there might be a relation between the strength of dielectric relaxations on MS spectrin and the deformability and flicker of RBC membrane.

Keywords: Dielectric relaxation; Erythrocyte deformability; RBC flicker; Spectrin skeleton.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Almizraq R, Tchir JDR, Acker J, Holovati JL (2012) The effects of biochemical rejuvenation on red blood cell microvesiculation, phosphatidylserine and CD47 expression during hypothermic storage. Cryobiology 65(3):349–350
    1. Anderson RA, Lovrein R (1981) Lectins utilize glycophorin in cytoskeletal control of human erythrocyte discocyte-echinocyte equilibria. Prog Clin Biol Res 56:207–229 - PubMed
    1. Anong WA, Franco T, Chu H, Weis TL, Devlin EE, Bodine DM, An X, Mohandas N, Low PS (2009) Adducin forms a bridge between the erythrocyte membrane and its cytoskeleton and regulates membrane cohesion. Blood 114:1904–1912 - PubMed - PMC
    1. Ballas SK, Mohandas N, Marton LJ, Shohet SB (1983) Cell Biology Stabilization of erythrocyte membranes by polyamines. Proc Nati Acad Sci USA 80:1942–1946
    1. Bao JZ, Davis CC, Swicord ML (1994) Microwave dielectric measurements of erythrocyte suspensions. Biophys J 66:2173–2180 - PubMed - PMC

MeSH terms

LinkOut - more resources