Urban ponds as hotspots of antibiotic resistome in the urban environment

doi:10.1016/j.jhazmat.2020.124008

. 2021 Feb 5:403:124008.

doi: 10.1016/j.jhazmat.2020.124008. Epub 2020 Sep 18.

Urban ponds as hotspots of antibiotic resistome in the urban environment

Liyuan Hou¹, Lanping Zhang², Furun Li³, Sijun Huang⁴, Jun Yang⁵, Cong Ma⁶, Duanxin Zhang⁷, Chang-Ping Yu⁸, Anyi Hu⁹

Affiliations

¹ CAS Key Laboratory of Urban pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China; Department of Chemistry, SUNY College of Environmental Science and Forestry, Syracuse, NY 13210, USA.
² CAS Key Laboratory of Urban pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China; University of Chinese Academy of Sciences, Beijing 100049, China.
³ CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, Guangdong, China; University of Chinese Academy of Sciences, Beijing 100049, China.
⁴ CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, Guangdong, China.
⁵ Aquatic EcoHealth Group, Fujian Key Laboratory of Watershed Ecology, Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China.
⁶ Xiamen Municipal Environment Technology Co., Ltd., Xiamen 361001, China.
⁷ General Water of Xiamen Sewage Co., Ltd., Xiamen 361001, China.
⁸ CAS Key Laboratory of Urban pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China; Graduate Institute of Environmental Engineering, National Taiwan University, Taipei 106, Taiwan.
⁹ CAS Key Laboratory of Urban pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China. Electronic address: ayhu@iue.ac.cn.

PMID: 33265037
DOI: 10.1016/j.jhazmat.2020.124008

Urban ponds as hotspots of antibiotic resistome in the urban environment

Liyuan Hou et al. J Hazard Mater. 2021.

. 2021 Feb 5:403:124008.

doi: 10.1016/j.jhazmat.2020.124008. Epub 2020 Sep 18.

Authors

Liyuan Hou¹, Lanping Zhang², Furun Li³, Sijun Huang⁴, Jun Yang⁵, Cong Ma⁶, Duanxin Zhang⁷, Chang-Ping Yu⁸, Anyi Hu⁹

Affiliations

¹ CAS Key Laboratory of Urban pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China; Department of Chemistry, SUNY College of Environmental Science and Forestry, Syracuse, NY 13210, USA.
² CAS Key Laboratory of Urban pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China; University of Chinese Academy of Sciences, Beijing 100049, China.
³ CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, Guangdong, China; University of Chinese Academy of Sciences, Beijing 100049, China.
⁴ CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, Guangdong, China.
⁵ Aquatic EcoHealth Group, Fujian Key Laboratory of Watershed Ecology, Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China.
⁶ Xiamen Municipal Environment Technology Co., Ltd., Xiamen 361001, China.
⁷ General Water of Xiamen Sewage Co., Ltd., Xiamen 361001, China.
⁸ CAS Key Laboratory of Urban pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China; Graduate Institute of Environmental Engineering, National Taiwan University, Taipei 106, Taiwan.
⁹ CAS Key Laboratory of Urban pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China. Electronic address: ayhu@iue.ac.cn.

PMID: 33265037
DOI: 10.1016/j.jhazmat.2020.124008

Abstract

The occurrence, dissemination and assembly processes of antibiotic resistance genes (ARGs) in urban water ecosystems are far from being understood. Here, we examined the diversity and abundance of ARGs in urban water ecosystems including landscape ponds, drinking water reservoirs, influents (IFs) and effluents (EFs) of wastewater treatment plants of a coastal city, China through high-throughput quantitative PCR. A total of 237 ARGs were identified, where multidrug, aminoglycoside and beta-lactamase resistance genes were the most abundant. Urban ponds had a comparatively high diversity and large numbers of shared ARGs with IFs and EFs. The average absolute abundance of ARGs (1.38 × 10⁷ copies/mL) and mobile genetic elements (MGEs) (4.19 × 10⁶ copies/mL) in ponds were only one order of magnitude lower than those of IFs, but higher than those of EFs and reservoirs. Stochastic processes dominated the ARG community assembly in IFs and ponds due to the random horizontal gene transfer caused by MGEs. These results imply that urban ponds are hotspots of ARGs. We further identified 25, 3, and 11 indicator ARGs for tracing the ARG contamination from IFs, EFs and ponds, respectively. Our study represents the first to highlight the role of urban ponds in the dissemination of ARGs.

Keywords: Antibiotic resistance genes; Bacterial community; Ecological processes; High-throughput quantitative PCR; Urban water ecosystem.

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Urban ponds as hotspots of antibiotic resistome in the urban environment

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Urban ponds as hotspots of antibiotic resistome in the urban environment

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