doi: 10.1016/0076-6879(87)54076-9.
5-Fluoroorotic acid as a selective agent in yeast molecular genetics
- PMID: 3323810
- DOI: 10.1016/0076-6879(87)54076-9
Item in Clipboard
5-Fluoroorotic acid as a selective agent in yeast molecular genetics
Methods Enzymol.
1987.
Abstract
5-FOA is an extremely useful reagent for the selection of Ura- cells amid a population of Ura+ cells. The selection is effective in transformation and recombination studies where loss of URA3+ is desired. A new plasmid shuffling procedure based on the 5-FOAR selection permits the recovery of conditional lethal mutations in cloned genes that encode vital functions.
Similar articles
-
New shuttle vectors for direct cloning in Saccharomyces cerevisiae.Gene. 1991 Jul 31;104(1):99-102. doi: 10.1016/0378-1119(91)90472-n. Gene. 1991. PMID: 1916284
-
Identification of the orotidine-5'-phosphate decarboxylase gene and development of a transformation system in the yeast Saccharomyces exiguus Yp74L-3.Biosci Biotechnol Biochem. 1998 Nov;62(11):2280-2. doi: 10.1271/bbb.62.2280. Biosci Biotechnol Biochem. 1998. PMID: 9972254
-
Transformation of the yeast Saccharomyces kluyveri by Saccharomyces cerevisiae-based plasmids.FEMS Microbiol Lett. 1991 Aug 1;66(2):149-52. doi: 10.1016/0378-1097(91)90324-4. FEMS Microbiol Lett. 1991. PMID: 1936943
-
Yeast vectors for integration at the HO locus.Nucleic Acids Res. 2001 Jun 15;29(12):E59-9. doi: 10.1093/nar/29.12.e59. Nucleic Acids Res. 2001. PMID: 11410682 Free PMC article.
-
Salinity tolerance--mechanisms, models and the metabolic engineering of complex traits.Genet Eng (N Y). 1998;20:153-76. doi: 10.1007/978-1-4899-1739-3_9. Genet Eng (N Y). 1998. PMID: 9666560 Review. No abstract available.
Cited by
-
PP2A(Cdc55) regulates G1 cyclin stability.Cell Cycle. 2013 Apr 15;12(8):1201-10. doi: 10.4161/cc.24231. Epub 2013 Mar 21. Cell Cycle. 2013. PMID: 23518505 Free PMC article.
-
A roadmap for gene system development in Clostridium.Anaerobe. 2016 Oct;41:104-112. doi: 10.1016/j.anaerobe.2016.05.011. Epub 2016 May 24. Anaerobe. 2016. PMID: 27234263 Free PMC article. Review.
-
Fungi of the order Mucorales express a "sealing-only" tRNA ligase.bioRxiv [Preprint]. 2023 Nov 17:2023.11.16.567474. doi: 10.1101/2023.11.16.567474. bioRxiv. 2023. Update in: RNA. 2024 Mar 18;30(4):354-366. doi: 10.1261/rna.079957.124. PMID: 38014270 Free PMC article. Updated. Preprint.
-
A novel small molecule RAD51 inactivator overcomes imatinib-resistance in chronic myeloid leukaemia.EMBO Mol Med. 2013 Mar;5(3):353-65. doi: 10.1002/emmm.201201760. Epub 2013 Jan 22. EMBO Mol Med. 2013. PMID: 23341130 Free PMC article.
-
RNA polymerase II carboxyl-terminal domain phosphorylation regulates protein stability of the Set2 methyltransferase and histone H3 di- and trimethylation at lysine 36.J Biol Chem. 2012 Jan 27;287(5):3249-56. doi: 10.1074/jbc.M111.273953. Epub 2011 Dec 7. J Biol Chem. 2012. PMID: 22157004 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
