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. 2020 Nov 18;10(1):20071.
doi: 10.1038/s41598-020-77068-0.

Characterization of miR-200 family members as blood biomarkers for human and laying hen ovarian cancer

Pui-Wah Choi  1   2   3 , Abbas Bahrampour  4   5 , Shu-Kay Ng  4 , Sze Kei Liu  3 , Wei Qiu  6 , Fang Xie  6 , Winston Patrick Kuo  6 , Joseph Kwong  7 , Karen H Hales  8 , Dale B Hales  8   9 , Kwong-Kwok Wong  10 , Errol R Norwitz  11 , Chun Kin Chow  12 , Ross S Berkowitz  1 , Shu-Wing Ng  13   14
Affiliations

Characterization of miR-200 family members as blood biomarkers for human and laying hen ovarian cancer

Pui-Wah Choi et al. Sci Rep. .

Abstract

MicroRNA-200 (miR-200) family is highly expressed in ovarian cancer. We evaluated the levels of family members relative to the internal control miR-103a in ovarian cancer and control blood specimens collected from American and Hong Kong Chinese institutions, as well as from a laying hen spontaneous ovarian cancer model. The levels of miR-200a, miR-200b and miR-200c were significantly elevated in all human cancer versus all control blood samples. Further analyses showed significantly higher miR-200 levels in Chinese control (except miR-429) and cancer (except miR-200a and miR141) samples than their respective American counterparts. Subtype-specific analysis showed that miR-200b had an overall elevated level in serous cancer compared with controls, whereas miR-429 was significantly elevated in clear cell and endometrioid cancer versus controls. MiR-429 was also significantly elevated in cancer versus control in laying hen plasma samples, consistent with the fact that endometrioid tumor is the prevalent type in this species. A neural network model consisting of miR-200a/200b/429/141 showed an area under the curve (AUC) value of 0.904 for American ovarian cancer prediction, whereas a model consisting of miR-200b/200c/429/141 showed an AUC value of 0.901 for Chinese women. Hence, miR-200 is informative as blood biomarkers for both human and laying hen ovarian cancer.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Figure 1
Figure 1
Receiver operating characteristic (ROC) curves for the best multilayer perceptron neural network predictive models to distinguish cancers from non-cancer controls for (a) American patients; and (b) Chinese patients.
See this image and copyright information in PMC

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References

    1. Reid, F. The World Ovarian Cancer Coalition Atlas- Global Trends in Incidence, Mortality and Survival. (World Ovarian Cancer Coalition, 2018).
    1. Vogell A, Evans ML. Cancer Screening in Women. Obstet. Gynecol. Clin. N. Am. 2019;46:485–499. doi: 10.1016/j.ogc.2019.04.007. - DOI - PubMed
    1. Siegel RL, Miller KD, Jemal A. Cancer statistics, 2019. CA A Cancer J. Clin. 2019;69:7–34. doi: 10.3322/caac.21551. - DOI - PubMed
    1. Lu KH. Screening for ovarian cancer in asymptomatic women. JAMA. 2018;319:557–558. doi: 10.1001/jama.2017.21894. - DOI - PubMed
    1. Dochez V, et al. Biomarkers and algorithms for diagnosis of ovarian cancer: CA125, HE4, RMI and ROMA, a review. J. Ovarian Res. 2019;12:28. doi: 10.1186/s13048-019-0503-7. - DOI - PMC - PubMed

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