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Review
. 2020 Dec:58:124-129.
doi: 10.1016/j.mib.2020.10.002. Epub 2020 Oct 25.

Intestinal organoid/enteroid-based models for Cryptosporidium

Seema Bhalchandra  1 , Hymlaire Lamisere  2 , Honorine Ward  3
Affiliations
Review

Intestinal organoid/enteroid-based models for Cryptosporidium

Seema Bhalchandra et al. Curr Opin Microbiol. 2020 Dec.

Abstract

Cryptosporidium is a leading cause of diarrhea and death in young children and untreated AIDS patients in resource-poor settings, and of waterborne outbreaks of disease in developed countries. However, there is no consistently effective treatment for vulnerable populations. Progress towards development of therapeutics for cryptosporidiosis has been hampered by lack of optimal culture systems to study it. New advances in organoid/enteroid technology have contributed to improved platforms to culture and propagate Cryptosporidium. Here we discuss recent breakthroughs in the field and highlight different models for functional ex vivo organoid or enteroidderived culture systems. These systems will lead to a better understanding of the mechanisms of host-parasite interactions in vivo.

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Figures

Figure 1
Figure 1
HIE models of C. parvum infection: (a) Intact 3D HIEs grown in Matrigel, are fragmented, incubated with C. parvum and re-plated in fresh Matrigel. Alternatively, HIE are dissociated into single cells and grown as 2D monolayers in (b) micro-titer plates or (c) transwell filters and infected apically with C. parvum oocysts or filtered sporozoites. (d) 3D silk scaffolds with luminal HIE monolayers are infected by introducing C. parvum oocysts or purified sporozoites into the lumen. Figure (d) modified and reproduced from Ref. [19] with permission from the American Society for Microbiology. Figure created in part with BioRender.com.
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