Canine Bone Marrow Mesenchymal Stem Cell Conditioned Media Affect Bacterial Growth, Biofilm-Associated Staphylococcus aureus and AHL-Dependent Quorum Sensing

doi:10.3390/microorganisms8101478

. 2020 Sep 26;8(10):1478.

doi: 10.3390/microorganisms8101478.

Canine Bone Marrow Mesenchymal Stem Cell Conditioned Media Affect Bacterial Growth, Biofilm-Associated Staphylococcus aureus and AHL-Dependent Quorum Sensing

Dobroslava Bujňáková¹, Anna Čuvalová¹, Milan Čížek², Filip Humenik², Michel Salzet^{3

4}, Daša Čížková^{2

3}

Affiliations

¹ Institute of Animal Physiology, Centre of Biosciences of the Slovak Academy of Sciences, 040 01 Košice, Slovakia.
² Center for Experimental and Clinical Regenerative Medicine, University of Veterinary Medicine and Pharmacy in Košice, 040 01 Košice, Slovakia.
³ Université Lille, Inserm, CHRU Lille, U-1192-Laboratoire Protéomique, Réponse Inflammatoire et Spectrométrie de Masse-PRISM, F-59000 Lille, France.
⁴ Institut Universitaire de France, 75000 Paris, France.

PMID: 32993120
PMCID: PMC7600979
DOI: 10.3390/microorganisms8101478

Canine Bone Marrow Mesenchymal Stem Cell Conditioned Media Affect Bacterial Growth, Biofilm-Associated Staphylococcus aureus and AHL-Dependent Quorum Sensing

Dobroslava Bujňáková et al. Microorganisms. 2020.

. 2020 Sep 26;8(10):1478.

doi: 10.3390/microorganisms8101478.

Authors

Dobroslava Bujňáková¹, Anna Čuvalová¹, Milan Čížek², Filip Humenik², Michel Salzet^{3

4}, Daša Čížková^{2

3}

Affiliations

¹ Institute of Animal Physiology, Centre of Biosciences of the Slovak Academy of Sciences, 040 01 Košice, Slovakia.
² Center for Experimental and Clinical Regenerative Medicine, University of Veterinary Medicine and Pharmacy in Košice, 040 01 Košice, Slovakia.
³ Université Lille, Inserm, CHRU Lille, U-1192-Laboratoire Protéomique, Réponse Inflammatoire et Spectrométrie de Masse-PRISM, F-59000 Lille, France.
⁴ Institut Universitaire de France, 75000 Paris, France.

PMID: 32993120
PMCID: PMC7600979
DOI: 10.3390/microorganisms8101478

Abstract

The present study investigated the in vitro antibacterial, antibiofilm and anti-Quorum Sensing (anti-QS) activities of canine bone marrow mesenchymal stem cell-conditioned media (cBM MSC CM) containing all secreted factors <30 K, using a disc diffusion test (DDT), spectrophotometric Crystal Violet Assay (SCVA) and Bioluminescence Assay (BA) with QS-reporter Escherichia coli JM109 pSB1142. The results show a sample-specific bacterial growth inhibition (zones varied between 7-30 mm), statistically significant modulation of biofilm-associated Staphylococcus aureus and Escherichia coli bioluminescence (0.391 ± 0.062 in the positive control to the lowest 0.150 ± 0.096 in the experimental group, cf. 11,714 ± 1362 to 7753 ± 700, given as average values of absorbance A₅₅₀ ± SD versus average values of relative light units to growth RLU/A₅₅₀ ± SD). The proteomic analysis performed in our previous experiment revealed the presence of several substances with documented antibacterial, antibiofilm and immunomodulatory properties (namely, apolipoprotein B and D; amyloid-β peptide; cathepsin B; protein S100-A4, galectin 3, CLEC3A, granulin, transferrin). This study highlights that cBM MSC CM may represent an important new approach to managing biofilm-associated and QS signal molecule-dependent bacterial infections. To the best of our knowledge, there is no previous documentation of canine BM MSC CM associated with in vitro antibiofilm and anti-QS activity.

Keywords: Escherichia coli JM109 pSB1142; biofilm; mesenchymal stem cells; quorum sensing.

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Conflict of interest statement

The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.

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References

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[1] Sung D.K., Chang Y.S., Sung S.I., Yoo H.S., Ahn S.Y., Park W.S. Antibacterial Effect of Mesenchymal Stem Cells against Escherichia Coli Is Mediated by Secretion of Beta- Defensin- 2 via Toll- like Receptor 4 Signalling. Cell. Microbiol. 2016;18:424–436. doi: 10.1111/cmi.12522. - DOI - PMC - PubMed

[2] Sung D.K., Chang Y.S., Sung S.I., Yoo H.S., Ahn S.Y., Park W.S. Antibacterial Effect of Mesenchymal Stem Cells against Escherichia Coli Is Mediated by Secretion of Beta- Defensin- 2 via Toll- like Receptor 4 Signalling. Cell. Microbiol. 2016;18:424–436. doi: 10.1111/cmi.12522. - DOI - PMC - PubMed

[3] Johnson V., Webb T., Norman A., Coy J., Kurihara J., Regan D., Dow S. Activated Mesenchymal Stem Cells Interact with Antibiotics and Host Innate Immune Responses to Control Chronic Bacterial Infections. Sci. Rep. 2017;7:9575. doi: 10.1038/s41598-017-08311-4. - DOI - PMC - PubMed

[4] Johnson V., Webb T., Norman A., Coy J., Kurihara J., Regan D., Dow S. Activated Mesenchymal Stem Cells Interact with Antibiotics and Host Innate Immune Responses to Control Chronic Bacterial Infections. Sci. Rep. 2017;7:9575. doi: 10.1038/s41598-017-08311-4. - DOI - PMC - PubMed

[5] Prockop D.J. Marrow Stromal Cells as Stem Cells for Nonhematopoietic Tissues. Science. 1997;276:71–74. doi: 10.1126/science.276.5309.71. - DOI - PubMed

[6] Prockop D.J. Marrow Stromal Cells as Stem Cells for Nonhematopoietic Tissues. Science. 1997;276:71–74. doi: 10.1126/science.276.5309.71. - DOI - PubMed

[7] Pittenger M.F. Multilineage Potential of Adult Human Mesenchymal Stem Cells. Science. 1999;284:143–147. doi: 10.1126/science.284.5411.143. - DOI - PubMed

[8] Pittenger M.F. Multilineage Potential of Adult Human Mesenchymal Stem Cells. Science. 1999;284:143–147. doi: 10.1126/science.284.5411.143. - DOI - PubMed

[9] Gupta N., Krasnodembskaya A., Kapetanaki M., Mouded M., Tan X., Serikov V., Matthay M.A. Mesenchymal Stem Cells Enhance Survival and Bacterial Clearance in Murine Escherichia Coli Pneumonia. Thorax. 2012;67:533–539. doi: 10.1136/thoraxjnl-2011-201176. - DOI - PMC - PubMed

[10] Gupta N., Krasnodembskaya A., Kapetanaki M., Mouded M., Tan X., Serikov V., Matthay M.A. Mesenchymal Stem Cells Enhance Survival and Bacterial Clearance in Murine Escherichia Coli Pneumonia. Thorax. 2012;67:533–539. doi: 10.1136/thoraxjnl-2011-201176. - DOI - PMC - PubMed

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Canine Bone Marrow Mesenchymal Stem Cell Conditioned Media Affect Bacterial Growth, Biofilm-Associated Staphylococcus aureus and AHL-Dependent Quorum Sensing

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Canine Bone Marrow Mesenchymal Stem Cell Conditioned Media Affect Bacterial Growth, Biofilm-Associated Staphylococcus aureus and AHL-Dependent Quorum Sensing

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Conflict of interest statement

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Abstract

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