Circadian Regulation of GluA2 mRNA Processing in the Rat Suprachiasmatic Nucleus and Other Brain Structures
- PMID: 32964314
- DOI: 10.1007/s12035-020-02141-8
Circadian Regulation of GluA2 mRNA Processing in the Rat Suprachiasmatic Nucleus and Other Brain Structures
Abstract
The mammalian circadian system consists of a major circadian pacemaker located in the suprachiasmatic nucleus (SCN) of the hypothalamus and peripheral clocks in the body, including brain structures. The SCN depends on glutamatergic neurotransmission for transmitting signals from the retina, and it exhibits spontaneous 24-h rhythmicity in neural activity. The aim of this work was to evaluate the degree and circadian rhythmicity of AMPA receptor GluA2 subunit R/G editing and alternative flip/flop splicing in the SCN and other brain structures in Wistar rats. Our data show that the circadian rhythmicity in the SCN's GluA2 mRNA level was highest at dawn, while the circadian rhythm in R/G editing peaked at CT10 and the rhythmic flip varied with the acrophase at the late subjective night. The circadian rhythmicity was confirmed for R/G editing and splicing in the CA3 hippocampal area, and rhythmic variation of the flip isoform was also measured in the olfactory bulbs and cerebellum. The correlations between the R/G editing and alternative flip/flop splicing revealed a structure-dependent direction. In the hippocampus, the edited (G)-form level was positively correlated with the flip variant abundance, in accord with published data; by contrast, in the SCN, the flip variant was in associated more with the unedited (R) form. The edited (G) form and flop isoform also predominated in the retina and cerebellum.
Keywords: Circadian clock; GluA2 subunit; R/G editing; Rat; Suprachiasmatic nucleus.
Similar articles
-
Modulation of dendritic AMPA receptor mRNA trafficking by RNA splicing and editing.Nucleic Acids Res. 2013 Jan 7;41(1):617-31. doi: 10.1093/nar/gks1223. Epub 2012 Nov 19. Nucleic Acids Res. 2013. PMID: 23166306 Free PMC article.
-
RNA editing (R/G site) and flip-flop splicing of the AMPA receptor subunit GluR2 in nervous tissue of epilepsy patients.Neurobiol Dis. 2004 Mar;15(2):371-9. doi: 10.1016/j.nbd.2003.11.006. Neurobiol Dis. 2004. PMID: 15006707
-
Gradients in the circadian expression of Per1 and Per2 genes in the rat suprachiasmatic nucleus.Eur J Neurosci. 2002 Apr;15(7):1153-62. doi: 10.1046/j.1460-9568.2002.01955.x. Eur J Neurosci. 2002. PMID: 11982626
-
Daily and circadian expression of neuropeptides in the suprachiasmatic nuclei of nocturnal and diurnal rodents.Brain Res Mol Brain Res. 2004 May 19;124(2):143-51. doi: 10.1016/j.molbrainres.2004.01.010. Brain Res Mol Brain Res. 2004. PMID: 15135222
-
Circadian profile of Per gene mRNA expression in the suprachiasmatic nucleus, paraventricular nucleus, and pineal body of aged rats.J Neurosci Res. 2001 Dec 15;66(6):1133-9. doi: 10.1002/jnr.10010. J Neurosci Res. 2001. PMID: 11746446
Cited by
-
The Circadian System Is Essential for the Crosstalk of VEGF-Notch-mediated Endothelial Angiogenesis in Ischemic Stroke.Neurosci Bull. 2023 Sep;39(9):1375-1395. doi: 10.1007/s12264-023-01042-9. Epub 2023 Mar 2. Neurosci Bull. 2023. PMID: 36862341 Free PMC article.
-
Translating around the clock: Multi-level regulation of post-transcriptional processes by the circadian clock.Cell Signal. 2021 Apr;80:109904. doi: 10.1016/j.cellsig.2020.109904. Epub 2020 Dec 25. Cell Signal. 2021. PMID: 33370580 Free PMC article. Review.
References
-
- Hastings MH, Maywood ES, Brancaccio M (2018) Generation of circadian rhythms in the suprachiasmatic nucleus. Nat Rev Neurosci 19(8):453–469. https://doi.org/10.1038/s41583-018-0026-z - DOI - PubMed
-
- Rath MF, Rohde K, Møller M (2012) Circadian oscillations of molecular clock components in the cerebellar cortex of the rat. Chronobiol Int 29(10):1289–1299. https://doi.org/10.3109/07420528.2012.728660 - DOI - PubMed
-
- Bering T, Carstensen MB, Wörtwein G, Weikop P, Rath MF (2018) The circadian oscillator of the cerebral cortex: molecular, biochemical and behavioral effects of deleting the Arntl clock gene in cortical neurons. Cereb Cortex 28(2):644–657. https://doi.org/10.1093/cercor/bhw406 - DOI - PubMed
-
- Jilg A, Lesny S, Peruzki N, Schwegler H, Selbach O, Dehghani F, Stehle JH (2010) Temporal dynamics of mouse hippocampal clock gene expression support memory processing. Hippocampus 20(3):377–388. https://doi.org/10.1002/hipo.20637 - DOI - PubMed
-
- Besharse JC, McMahon DG (2016) The retina and other light-sensitive ocular clocks. J Biol Rhythm 31(3):223–243. https://doi.org/10.1177/0748730416642657 - DOI
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Miscellaneous