Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Jan;58(1):439-449.
doi: 10.1007/s12035-020-02141-8. Epub 2020 Sep 22.

Circadian Regulation of GluA2 mRNA Processing in the Rat Suprachiasmatic Nucleus and Other Brain Structures

Affiliations

Circadian Regulation of GluA2 mRNA Processing in the Rat Suprachiasmatic Nucleus and Other Brain Structures

Hana Míková et al. Mol Neurobiol. 2021 Jan.

Abstract

The mammalian circadian system consists of a major circadian pacemaker located in the suprachiasmatic nucleus (SCN) of the hypothalamus and peripheral clocks in the body, including brain structures. The SCN depends on glutamatergic neurotransmission for transmitting signals from the retina, and it exhibits spontaneous 24-h rhythmicity in neural activity. The aim of this work was to evaluate the degree and circadian rhythmicity of AMPA receptor GluA2 subunit R/G editing and alternative flip/flop splicing in the SCN and other brain structures in Wistar rats. Our data show that the circadian rhythmicity in the SCN's GluA2 mRNA level was highest at dawn, while the circadian rhythm in R/G editing peaked at CT10 and the rhythmic flip varied with the acrophase at the late subjective night. The circadian rhythmicity was confirmed for R/G editing and splicing in the CA3 hippocampal area, and rhythmic variation of the flip isoform was also measured in the olfactory bulbs and cerebellum. The correlations between the R/G editing and alternative flip/flop splicing revealed a structure-dependent direction. In the hippocampus, the edited (G)-form level was positively correlated with the flip variant abundance, in accord with published data; by contrast, in the SCN, the flip variant was in associated more with the unedited (R) form. The edited (G) form and flop isoform also predominated in the retina and cerebellum.

Keywords: Circadian clock; GluA2 subunit; R/G editing; Rat; Suprachiasmatic nucleus.

PubMed Disclaimer

Similar articles

Cited by

References

    1. Hastings MH, Maywood ES, Brancaccio M (2018) Generation of circadian rhythms in the suprachiasmatic nucleus. Nat Rev Neurosci 19(8):453–469. https://doi.org/10.1038/s41583-018-0026-z - DOI - PubMed
    1. Rath MF, Rohde K, Møller M (2012) Circadian oscillations of molecular clock components in the cerebellar cortex of the rat. Chronobiol Int 29(10):1289–1299. https://doi.org/10.3109/07420528.2012.728660 - DOI - PubMed
    1. Bering T, Carstensen MB, Wörtwein G, Weikop P, Rath MF (2018) The circadian oscillator of the cerebral cortex: molecular, biochemical and behavioral effects of deleting the Arntl clock gene in cortical neurons. Cereb Cortex 28(2):644–657. https://doi.org/10.1093/cercor/bhw406 - DOI - PubMed
    1. Jilg A, Lesny S, Peruzki N, Schwegler H, Selbach O, Dehghani F, Stehle JH (2010) Temporal dynamics of mouse hippocampal clock gene expression support memory processing. Hippocampus 20(3):377–388. https://doi.org/10.1002/hipo.20637 - DOI - PubMed
    1. Besharse JC, McMahon DG (2016) The retina and other light-sensitive ocular clocks. J Biol Rhythm 31(3):223–243. https://doi.org/10.1177/0748730416642657 - DOI

LinkOut - more resources