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. 2020;152(1 & 2):108-110.
doi: 10.4103/ijmr.IJMR_3121_20.

Utility of a modified heat inactivation method for direct detection of SARS-CoV-2 by RT-qPCR in viral transport medium bypassing RNA extraction: A preliminary study

Kalichami Alagarasu  1 , Varsha A Potdar  2 , Veena Vipat  2 , Supriya Hundekar  3 , Rashmi Gunjikar  4 , Manohar L Choudhary  2 , Priya Abraham  5 , Kavita S Lole  3
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Utility of a modified heat inactivation method for direct detection of SARS-CoV-2 by RT-qPCR in viral transport medium bypassing RNA extraction: A preliminary study

Kalichami Alagarasu et al. Indian J Med Res. 2020.
No abstract available

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None

Figures

Figure
Figure
Comparison of cycle threshold (Ct) values for different SARS-CoV-2 genes and internal control gene between samples subjected to protease/dithiothreitol/heat inactivation with and without dilution. The data are represented as aligned dot plot with lines for mean and standard error of the mean. For each gene, samples which showed Ct value in both treatments only were included for comparison. ***P<0.001 compared to without dilution.
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