Isolation and characterization of a beta-tubulin gene from Candida albicans
- PMID: 3290053
- DOI: 10.1016/0378-1119(88)90545-8
Isolation and characterization of a beta-tubulin gene from Candida albicans
Abstract
We report the isolation and nucleotide sequence determination of a beta-tubulin gene (TUB2) from the pathogenic dimorphic fungus Candida albicans. Nucleotide sequence analysis revealed that TUB2 encodes a protein of 449 amino acids (aa) with considerable sequence homology to beta-tubulins isolated from other fungal species. The nucleotide sequence of the C. albicans gene is 70% homologous to that of the Saccharomyces cerevisiae gene. The coding region for the C. albicans beta-tubulin gene is interrupted by two introns. The first intron occurs after the 4th aa and the second intron occurs after the 13th aa. A comparison with other fungal beta-tubulin genes indicates that the intron locations are highly conserved. Codon usage in the C. albicans TUB2 gene is nonrandom, as has been observed for other fungal beta-tubulin genes. The C. albicans TUB2 gene is transcribed to yield a 1.8-kb mRNA species. On the basis of genomic Southern-blot analysis, we conclude that C. albicans most likely possesses a single beta-tubulin gene.
Similar articles
-
Characterization of two divergent beta-tubulin genes from Colletotrichum graminicola.Gene. 1990 Feb 14;86(2):163-70. doi: 10.1016/0378-1119(90)90275-v. Gene. 1990. PMID: 2323570
-
Isolation and characterization of a gene encoding alpha-tubulin from Candida albicans.Gene. 1997 Mar 18;187(2):151-8. doi: 10.1016/s0378-1119(96)00737-8. Gene. 1997. PMID: 9099874
-
Characterization of alpha and beta tubulin genes in the dimorphic fungus Histoplasma capsulatum.J Gen Microbiol. 1989 Jul;135(7):1817-32. doi: 10.1099/00221287-135-7-1817. J Gen Microbiol. 1989. PMID: 2614394
-
[Isolation and molecular characterization of the CaPHO85 gene: a negative regulator of phosphate metabolism (PHO system) in Candida albicans].Nihon Ishinkin Gakkai Zasshi. 2003;44(2):101-5. doi: 10.3314/jjmm.44.101. Nihon Ishinkin Gakkai Zasshi. 2003. PMID: 12748591 Review. Japanese.
-
Developmental and molecular biology of switching in Candida albicans.Oral Surg Oral Med Oral Pathol. 1994 Aug;78(2):194-201. doi: 10.1016/0030-4220(94)90147-3. Oral Surg Oral Med Oral Pathol. 1994. PMID: 7936589 Review.
Cited by
-
Structural analysis of mutations in the Drosophila beta 2-tubulin isoform reveals regions in the beta-tubulin molecular required for general and for tissue-specific microtubule functions.Genetics. 1995 Jan;139(1):267-86. doi: 10.1093/genetics/139.1.267. Genetics. 1995. PMID: 7705629 Free PMC article.
-
Are tubulin isotypes functionally significant.Mol Biol Cell. 1993 May;4(5):445-57. doi: 10.1091/mbc.4.5.445. Mol Biol Cell. 1993. PMID: 8334301 Free PMC article. Review. No abstract available.
-
Overexpression of a cloned IMP dehydrogenase gene of Candida albicans confers resistance to the specific inhibitor mycophenolic acid.J Bacteriol. 1997 Apr;179(7):2331-8. doi: 10.1128/jb.179.7.2331-2338.1997. J Bacteriol. 1997. PMID: 9079920 Free PMC article.
-
Comparison of the separation of Candida albicans chromosome-sized DNA by pulsed-field gel electrophoresis techniques.Nucleic Acids Res. 1989 May 25;17(10):3783-93. doi: 10.1093/nar/17.10.3783. Nucleic Acids Res. 1989. PMID: 2660101 Free PMC article.
-
Molecular analysis of the Septoria nodorum beta-tubulin gene and characterization of a benomyl-resistance mutation.Mol Gen Genet. 1993 Feb;237(1-2):58-64. doi: 10.1007/BF00282784. Mol Gen Genet. 1993. PMID: 8455567
Publication types
MeSH terms
Substances
Associated data
- Actions
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
