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. 2020 Aug 27;13(17):3786.
doi: 10.3390/ma13173786.

Laser Texturing as a Way of Influencing the Micromechanical and Biological Properties of the Poly(L-Lactide) Surface

Magdalena Tomanik  1 , Magdalena Kobielarz  1 , Jarosław Filipiak  1 , Maria Szymonowicz  2 , Agnieszka Rusak  3 , Katarzyna Mroczkowska  4 , Arkadiusz Antończak  4 , Celina Pezowicz  1
Affiliations

Laser Texturing as a Way of Influencing the Micromechanical and Biological Properties of the Poly(L-Lactide) Surface

Magdalena Tomanik et al. Materials (Basel). .

Abstract

Laser-based technologies are extensively used for polymer surface patterning and/or texturing. Different micro- and nanostructures can be obtained thanks to a wide range of laser types and beam parameters. Cell behavior on various types of materials is an extensively investigated phenomenon in biomedical applications. Polymer topography such as height, diameter, and spacing of the patterning will cause different cell responses, which can also vary depending on the utilized cell types. Structurization can highly improve the biological performance of the material without any need for chemical modification. The aim of the study was to evaluate the effect of CO2 laser irradiation of poly(L-lactide) (PLLA) thin films on the surface microhardness, roughness, wettability, and cytocompatibility. The conducted testing showed that CO2 laser texturing of PLLA provides the ability to adjust the structural and physical properties of the PLLA surface to the requirements of the cells despite significant changes in the mechanical properties of the laser-treated surface polymer.

Keywords: cytotoxicity; laser irradiation; micromechanical properties; poly(L-lactide); surface enhancement.

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Conflict of interest statement

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Figure 1
Figure 1
Microscopic images of the obtained surfaces (magnification 100×) recorded for reference material (Ref) and laser treated with accumulated fluences of 24 J/cm2 (F1), 48 J/cm2 (F2) and 71 J/cm2 (F3).
Figure 2
Figure 2
The wettability of the surface modified with different laser parameters; statistical significance between the study groups for water and PBS, obtained in one-way ANOVA and post-hoc Tukey’s tests; * p < 0.001.
Figure 3
Figure 3
Culture of Balb/3T3 fibroblasts after 24-h contact with the test materials around and under the surface of the specimen: (A) and (B) Ref, (C) and (D) F1, (E) and (F) F2, (G) and (H) F3, and (I) control cell culture without contact with materials. Altered cells occur only in a limited zone under the specimens. Magnification: ×100.
Figure 4
Figure 4
Culture of Balb/3T3 fibroblasts 24 h after application of the cells on the surfaces of the materials: (A) Ref, (B) F1, (C) F2, (D) F3, and (E) control cell culture without contact with the material No cell colonization of the material surfaces was observed. Magnification: ×100.
Figure 5
Figure 5
Typical loads vs. indentation depth curves for reference and irradiated PLLA specimens.
See this image and copyright information in PMC

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