Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Apr;36(3):681-690.
doi: 10.1007/s10103-020-03131-x. Epub 2020 Aug 19.

Effects of photobiomodulation on cellular viability and cancer stem cell phenotype in oral squamous cell carcinoma

Ana Melissa Ccopa Ibarra  1 , Mônica Pereira Garcia  1 , Marlene Ferreira  1 , Daniela de Fátima Teixeira da Silva  1 , Christiane Pavani  1 , Raquel Agnelli Mesquita-Ferrari  1 , Kristianne Porta Santos Fernandes  1 , Fabio Daumas Nunes  2 , Maria Fernanda Setúbal Destro Rodrigues  3   4
Affiliations

Effects of photobiomodulation on cellular viability and cancer stem cell phenotype in oral squamous cell carcinoma

Ana Melissa Ccopa Ibarra et al. Lasers Med Sci. 2021 Apr.

Abstract

Oral squamous cell carcinoma (OSCC) is the most common head and neck malignancy; it has been shown that cancer stem cells (CSC) are present in OSCC and associated with tumor growth, invasion, metastasis, and therapeutic resistance. Photobiomodulation (PBM) is an alternative tool for oncologic treatment adverse effects such as oral mucositis (OM); however, controversy exists regarding the undesirable effects of PBM on tumor or CSC. This study aimed to evaluate in vitro, the effects of PBM, with the same dosimetric parameters as those used in the clinic for OM prevention and treatment, on OSCC cellular viability, as well as PBM's effect on CSC properties and its phenotype. OSCC cell lines were submitted to single or daily PBM with 3 J/cm2 and 6 J/cm2 and then the cellular viability was evaluated by MTT, NRU (neutral red uptake), and CVS (crystal violet staining). The CSC populations were evaluated by clonogenic formation assay, flow cytometry, and RT-qPCR. The single PBM with the 3 J/cm2 group was associated with increased cellular viability. Daily PBM with 3 J/cm2 and 6 J/cm2 was associated with a significant decrease in cellular viability. Additionally, daily PBM was not able to promote CSC self-renewal or the CD44high/ESAlow and CD44high/ESAhigh cellular phenotypes. Moreover, a decrease in the number of spheres and in the expression of the CSC related gene BMI1 was observed after daily PBM with 6 J/cm2. Daily PBM with 3 J/cm2 and 6 J/cm2 showed an inhibitory effect on cellular viability and was not able to promote the CSC self-renewal or phenotype.

Keywords: Cancer stem cells; Low-level laser therapy; Oral squamous cell carcinoma; Photobiomodulation.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Duprez F, Berwouts D, De Neve W, Bonte K, Boterberg T, Deron P, Huvenne W, Rottery S, Mareel M (2017) Distant metastases in head and neck cancer. Head Neck 39:1733–1743. https://doi.org/10.1002/hed.24687 - DOI - PubMed
    1. de Vicente JC, Rodríguez-Santamarta T, Rosado P, Peña I, de Villalaín L (2012) Survival after free flap reconstruction in patients with advanced oral squamous cell carcinoma. J Oral Maxillofac Surg 70:453–459. https://doi.org/10.1016/j.joms.2011.02.020 - DOI - PubMed
    1. Grégoire V, Lefebvre J-L, Licitra L, Felip E, Group ObotEEEGW (2010) Squamous cell carcinoma of the head and neck: EHNS–ESMO–ESTRO Clinical Practice Guidelines for diagnosis, treatment and follow-up. Ann Oncol 21:184–186. https://doi.org/10.1093/annonc/mdq185 - DOI
    1. Vermorken JB, Specenier P (2010) Optimal treatment for recurrent/metastatic head and neck cancer. Ann Oncol 21:252–261. https://doi.org/10.1093/annonc/mdq453 - DOI
    1. Nör C, Zhang Z, Warner KA, Bernardi L, Visioli F, Helman JI, Roesler R, Nör JE (2014) Cisplatin induces Bmi-1 and Enhances the stem cell fraction in head and neck cancer. Neoplasia 16:137–138. https://doi.org/10.1593/neo.131744 - DOI - PubMed - PMC

MeSH terms

Grants and funding

LinkOut - more resources