Metagenome Sequences of a Wastewater Treatment Plant Digester Sludge-Derived Enrichment Culture

doi:10.1128/MRA.00712-20

. 2020 Aug 6;9(32):e00712-20.

doi: 10.1128/MRA.00712-20.

Metagenome Sequences of a Wastewater Treatment Plant Digester Sludge-Derived Enrichment Culture

Heiko Nacke¹, Laura L Kirck¹, Sophia Schwarz¹, Dominik Schneider¹, Anja Poehlein¹, Rolf Daniel²

Affiliations

¹ Genomic and Applied Microbiology and Göttingen Genomics Laboratory, Institute of Microbiology and Genetics, Georg-August University of Göttingen, Göttingen, Germany.
² Genomic and Applied Microbiology and Göttingen Genomics Laboratory, Institute of Microbiology and Genetics, Georg-August University of Göttingen, Göttingen, Germany rdaniel@gwdg.de.

PMID: 32763939
PMCID: PMC7409856
DOI: 10.1128/MRA.00712-20

Metagenome Sequences of a Wastewater Treatment Plant Digester Sludge-Derived Enrichment Culture

Heiko Nacke et al. Microbiol Resour Announc. 2020.

. 2020 Aug 6;9(32):e00712-20.

doi: 10.1128/MRA.00712-20.

Authors

Heiko Nacke¹, Laura L Kirck¹, Sophia Schwarz¹, Dominik Schneider¹, Anja Poehlein¹, Rolf Daniel²

Affiliations

¹ Genomic and Applied Microbiology and Göttingen Genomics Laboratory, Institute of Microbiology and Genetics, Georg-August University of Göttingen, Göttingen, Germany.
² Genomic and Applied Microbiology and Göttingen Genomics Laboratory, Institute of Microbiology and Genetics, Georg-August University of Göttingen, Göttingen, Germany rdaniel@gwdg.de.

PMID: 32763939
PMCID: PMC7409856
DOI: 10.1128/MRA.00712-20

Abstract

We sequenced the metagenome of a microbial community enriched under strictly anaerobic conditions from wastewater treatment plant-derived digester sludge. The metagenomic analysis of the enrichment revealed that Acetobacterium and methanogenic archaea belonged to the dominant prokaryotes, and genes encoding components of the Wood-Ljungdahl pathway were identified.

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References

1. Guo J, Peng Y, Ni BJ, Han X, Fan L, Yuan Z. 2015. Dissecting microbial community structure and methane-producing pathways of a full-scale anaerobic reactor digesting activated sludge from wastewater treatment by metagenomic sequencing. Microb Cell Fact 14:33. doi:10.1186/s12934-015-0218-4. - DOI - PMC - PubMed
1. Enzmann F, Mayer F, Rother M, Holtmann D. 2018. Methanogens: biochemical background and biotechnological applications. AMB Express 8:1. doi:10.1186/s13568-017-0531-x. - DOI - PMC - PubMed
1. Poehlein A, Cebulla M, Ilg MM, Bengelsdorf FR, Schiel-Bengelsdorf B, Whited G, Andreesen JR, Gottschalk G, Daniel R, Dürre P. 2015. The complete genome sequence of Clostridium aceticum: a missing link between Rnf- and cytochrome-containing autotrophic acetogens. mBio 6:e01168-15. doi:10.1128/mBio.01168-15. - DOI - PMC - PubMed
1. Chen S, Zhou Y, Chen Y, Gu J. 2018. Fastp: an ultra-fast all-in-one FASTQ preprocessor. Bioinformatics 34:i884–i890. doi:10.1093/bioinformatics/bty560. - DOI - PMC - PubMed
1. Menzel P, Ng KL, Krogh A. 2016. Fast and sensitive taxonomic classification for metagenomics with Kaiju. Nat Commun 7:11257. doi:10.1038/ncomms11257. - DOI - PMC - PubMed

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[1] Guo J, Peng Y, Ni BJ, Han X, Fan L, Yuan Z. 2015. Dissecting microbial community structure and methane-producing pathways of a full-scale anaerobic reactor digesting activated sludge from wastewater treatment by metagenomic sequencing. Microb Cell Fact 14:33. doi:10.1186/s12934-015-0218-4. - DOI - PMC - PubMed

[2] Guo J, Peng Y, Ni BJ, Han X, Fan L, Yuan Z. 2015. Dissecting microbial community structure and methane-producing pathways of a full-scale anaerobic reactor digesting activated sludge from wastewater treatment by metagenomic sequencing. Microb Cell Fact 14:33. doi:10.1186/s12934-015-0218-4. - DOI - PMC - PubMed

[3] Enzmann F, Mayer F, Rother M, Holtmann D. 2018. Methanogens: biochemical background and biotechnological applications. AMB Express 8:1. doi:10.1186/s13568-017-0531-x. - DOI - PMC - PubMed

[4] Enzmann F, Mayer F, Rother M, Holtmann D. 2018. Methanogens: biochemical background and biotechnological applications. AMB Express 8:1. doi:10.1186/s13568-017-0531-x. - DOI - PMC - PubMed

[5] Poehlein A, Cebulla M, Ilg MM, Bengelsdorf FR, Schiel-Bengelsdorf B, Whited G, Andreesen JR, Gottschalk G, Daniel R, Dürre P. 2015. The complete genome sequence of Clostridium aceticum: a missing link between Rnf- and cytochrome-containing autotrophic acetogens. mBio 6:e01168-15. doi:10.1128/mBio.01168-15. - DOI - PMC - PubMed

[6] Poehlein A, Cebulla M, Ilg MM, Bengelsdorf FR, Schiel-Bengelsdorf B, Whited G, Andreesen JR, Gottschalk G, Daniel R, Dürre P. 2015. The complete genome sequence of Clostridium aceticum: a missing link between Rnf- and cytochrome-containing autotrophic acetogens. mBio 6:e01168-15. doi:10.1128/mBio.01168-15. - DOI - PMC - PubMed

[7] Chen S, Zhou Y, Chen Y, Gu J. 2018. Fastp: an ultra-fast all-in-one FASTQ preprocessor. Bioinformatics 34:i884–i890. doi:10.1093/bioinformatics/bty560. - DOI - PMC - PubMed

[8] Chen S, Zhou Y, Chen Y, Gu J. 2018. Fastp: an ultra-fast all-in-one FASTQ preprocessor. Bioinformatics 34:i884–i890. doi:10.1093/bioinformatics/bty560. - DOI - PMC - PubMed

[9] Menzel P, Ng KL, Krogh A. 2016. Fast and sensitive taxonomic classification for metagenomics with Kaiju. Nat Commun 7:11257. doi:10.1038/ncomms11257. - DOI - PMC - PubMed

[10] Menzel P, Ng KL, Krogh A. 2016. Fast and sensitive taxonomic classification for metagenomics with Kaiju. Nat Commun 7:11257. doi:10.1038/ncomms11257. - DOI - PMC - PubMed

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Metagenome Sequences of a Wastewater Treatment Plant Digester Sludge-Derived Enrichment Culture

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Metagenome Sequences of a Wastewater Treatment Plant Digester Sludge-Derived Enrichment Culture

Authors

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Abstract

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