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. 2021 Jan:75:65-71.
doi: 10.1016/j.mri.2020.07.011. Epub 2020 Jul 28.

Significance of single and multi-voxel 1H magnetic resonance spectroscopy in the quantification of myocellular lipid in young non-obese Asian Indian males

Roshan Samuel Livingstone  1 , Abel Juhan Thomas  2 , Riddhi Dasgupta  3 , Shajith Anoop  3 , Mathews Edatharayil Kurian  3 , Meredith Hawkins  4 , Nihal Thomas  3
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Significance of single and multi-voxel 1H magnetic resonance spectroscopy in the quantification of myocellular lipid in young non-obese Asian Indian males

Roshan Samuel Livingstone et al. Magn Reson Imaging. 2021 Jan.

Abstract

To prospectively assess intramyocellular lipids (IMCL) and extramyocellular lipids (EMCL) using single voxel spectroscopy (SVS) and multi voxel magnetic resonance spectroscopy (MVS) in soleus muscle and correlate results with metabolic variables in non-obese (BMI < 23 kg/m2) Asian Indian males. Thirty one patients with diabetes (cases) and twelve normoglycaemic subjects (controls) underwent point resolved spectroscopy sequence (PRESS) of soleus muscle using SVS and MVS in a 3 T MRI scanner. Visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT) were measured from MRI images and body composition was measured from dual-energy x-ray absorptiometry (DXA). The mean IMCL from SVS and MVS were 1.6% and 2.6% in cases and 2.3% and 3.4% in controls respectively. The mean EMCL from SVS and MVS were 1.8% and 3% in cases and 1.5% and 3% respectively in controls. A significant correlation between IMCL and total fat mass (rho = 0.42, p < 0.01) and total body fat (rho = 0.46; p < 0.01) were observed in cases while using the SVS technique and no correlations were found in the MVS technique. The SVS showed significant correlations between total myocellular lipids with VAT and SAT in cases alone. Total myocellular lipids acquired using both techniques showed a significant correlation with BMI, waist circumference, total fat mass, total body fat and truncal fat in cases alone. Quantification of IMCL of soleus muscle using the SVS technique is useful in studying the relationship with metabolic markers in non-obese Asian Indians with diabetes.

Keywords: Body composition; Intramyocellular lipids; Magnetic resonance spectroscopy; Soleus muscle.

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Figures

Figure 1
Figure 1
(a) MR spectra of S. muscle acquired using SVS technique. (b) MR spectra of TA muscle acquired using SVS technique. Six resonance peaks in both muscles are defined: (a) IMCL -CH3 proton peak at 0.9ppm, (b) EMCL -CH3 proton peak at 1.1ppm, (c) IMCL-CH2 proton peak at 1.3ppm, (d) EMCL-CH2 proton peak at 1.5ppm, (e) total creatinine -CH3 peak at 3.0ppm and (f) the trimethylamines peak at 3.2 ppm.
Figure 2
Figure 2
MR spectra of S. muscle acquired using MVS technique. The arrows indicate the splitting of peaks and overlapping peaks.
See this image and copyright information in PMC

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