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. 2020:641:53-73.
doi: 10.1016/bs.mie.2020.04.036. Epub 2020 Jun 13.

Photo-releasable derivatives of inositol pyrophosphates

Sebastian Hauke  1 , Tamara Bittner  2 , Henning J Jessen  3 , Carsten Schultz  4
Affiliations

Photo-releasable derivatives of inositol pyrophosphates

Sebastian Hauke et al. Methods Enzymol. 2020.

Abstract

The specific non-invasive control of intracellular signaling events requires advanced tools that enter cells by diffusion and are controllable by light. Here, we detail the synthesis and application of membrane-permeant caged inositol pyrophosphates with respect to cell entry and cell distribution. We recently published the synthesis of these tools as well as their effect on PH-domain localization in HeLa cells and oscillations of the intracellular calcium concentration in β-cells, which are known to drive insulin secretion. In this chapter, we discuss the possibilities and limitations when using cell-penetrating inositol pyrophosphates. We provide a detailed protocol for the application in live mouse β-cells and we discuss the image analysis needed for following effects on calcium signaling.

Keywords: Cell entry; Inositol phosphate; Photo-activation; Prodrugs; Signaling.

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