Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Mar;236(3):1810-1821.
doi: 10.1002/jcp.29963. Epub 2020 Jul 23.

miR-142a-5p promoted osteoblast differentiation via targeting nuclear factor IA

Hairui Yuan  1 , Mengyue Li  1 , Xue Feng  1 , Endong Zhu  1 , Baoli Wang  1
Affiliations

miR-142a-5p promoted osteoblast differentiation via targeting nuclear factor IA

Hairui Yuan et al. J Cell Physiol. 2021 Mar.

Abstract

miR-142a-5p plays critical roles in multiple biological processes and diseases, such as inflammation and tumorigenesis. However, it remains to be explored if and how miR-142a-5p contributes to osteoblast differentiation. In this study, our results showed that miR-142a-5p was highly expressed in bone tissue of mice and increased during osteogenesis in preosteoblast MC3T3-E1 cells. Supplementing miR-142a-5p activity using miR-142a-5p agomir promoted osteogenic differentiation in stromal cell line ST2 and preosteoblastic line MC3T3-E1. Conversely, miR-142a-5p antagomir, an inhibitor of endogenous miR-142a-5p, could reduce osteoblast differentiation in ST2 and MC3T3-E1 cells. Nuclear factor IA (NFIA), a site-specific transcriptional factor, was demonstrated to be directly targeted by miR-142a-5p. Overexpression of NFIA inhibited miR-142a-5p-mediated osteoblast differentiation in ST2 cells. Furthermore, mechanism explorations revealed that Wnt/β-catenin signaling transcriptionally regulated the expression of miR-142a-5p during osteogenic differentiation. β-catenin binds to the T-cell factor/lymphoid enhancer factor binding motif within the promoter of miR-142 and positively regulates its transcriptional activity. Our findings suggested that miR-142a-5p promoted osteoblast differentiation via targeting NFIA.

Keywords: NFIA; Wnt/β-catenin signaling; miR-142a-5p; osteoblast differentiation.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

REFERENCES

    1. Artigas, N., Gamez, B., Cubillos-Rojas, M., Sanchez-de Diego, C., Valer, J. A., Pons, G., … Ventura, F. (2017). p53 inhibits SP7/Osterix activity in the transcriptional program of osteoblast differentiation. Cell Death and Differentiation, 24, 2022-2031.
    1. Atcha, F. A., Syed, A., Wu, B., Hoverter, N. P., Yokoyama, N. N., Ting, J. H., … Waterman, M. L. (2007). A unique DNA binding domain converts T-cell factors into strong Wnt effectors. Molecular and Cellular Biology, 27, 8352-8363.
    1. Bennett, C. N., Ouyang, H., Ma, Y. L., Zeng, Q., Gerin, I., Sousa, K. M., … MacDougald, O. A. (2007). Wnt10b increases postnatal bone formation by enhancing osteoblast differentiation. Journal of Bone and Mineral Research, 22, 1924-1932.
    1. Cadigan, K. M., & Waterman, M. L. (2012). TCF/LEFs and Wnt signaling in the nucleus. Cold Spring Harbor Perspectives in Biology, 4, a007906. https://doi.org/10.1101/cshperspect.a007906
    1. Chanda, S., Nandi, S., & Chawla-Sarkar, M. (2016). Rotavirus-induced miR-142-5p elicits proviral milieu by targeting non-canonical transforming growth factor beta signalling and apoptosis in cells. Cellular Microbiology, 18, 733-747.

Publication types

LinkOut - more resources