Modeling endodermal organ development and diseases using human pluripotent stem cell-derived organoids
- PMID: 32652003
- PMCID: PMC7683020
- DOI: 10.1093/jmcb/mjaa031
Modeling endodermal organ development and diseases using human pluripotent stem cell-derived organoids
Abstract
Recent advances in development of protocols for directed differentiation from human pluripotent stem cells (hPSCs) to defined lineages, in combination with 3D organoid technology, have facilitated the generation of various endoderm-derived organoids for in vitro modeling of human gastrointestinal development and associated diseases. In this review, we discuss current state-of-the-art strategies for generating hPSC-derived endodermal organoids including stomach, liver, pancreatic, small intestine, and colonic organoids. We also review the advantages of using this system to model various human diseases and evaluate the shortcomings of this technology. Finally, we emphasize how other technologies, such as genome editing and bioengineering, can be incorporated into the 3D hPSC-organoid models to generate even more robust and powerful platforms for understanding human organ development and disease modeling.
Keywords: disease modeling; endoderm; gastrointestinal development; human pluripotent stem cells.
© The Author(s) (2020). Published by Oxford University Press on behalf of Journal of Molecular Cell Biology, IBCB, SIBS, CAS.
Figures
Similar articles
-
In vitro generation of human pluripotent stem cell derived lung organoids.Elife. 2015 Mar 24;4:e05098. doi: 10.7554/eLife.05098. Elife. 2015. PMID: 25803487 Free PMC article.
-
Generation of human colonic organoids from human pluripotent stem cells.Methods Cell Biol. 2020;159:201-227. doi: 10.1016/bs.mcb.2020.03.001. Epub 2020 Apr 8. Methods Cell Biol. 2020. PMID: 32586443 Free PMC article.
-
Generation of Gastrointestinal Organoids from Human Pluripotent Stem Cells.Methods Mol Biol. 2017;1597:167-177. doi: 10.1007/978-1-4939-6949-4_12. Methods Mol Biol. 2017. PMID: 28361317
-
The endothelium, a key actor in organ development and hPSC-derived organoid vascularization.J Biomed Sci. 2020 May 22;27(1):67. doi: 10.1186/s12929-020-00661-y. J Biomed Sci. 2020. PMID: 32443983 Free PMC article. Review.
-
Disease Modeling Using 3D Organoids Derived from Human Induced Pluripotent Stem Cells.Int J Mol Sci. 2018 Mar 21;19(4):936. doi: 10.3390/ijms19040936. Int J Mol Sci. 2018. PMID: 29561796 Free PMC article. Review.
Cited by
-
Prostatic lineage differentiation from human embryonic stem cells through inducible expression of NKX3-1.Stem Cell Res Ther. 2024 Sep 2;15(1):274. doi: 10.1186/s13287-024-03886-y. Stem Cell Res Ther. 2024. PMID: 39218930 Free PMC article.
-
Transcriptional remodeling by OTX2 directs specification and patterning of mammalian definitive endoderm.bioRxiv [Preprint]. 2024 May 30:2024.05.30.596630. doi: 10.1101/2024.05.30.596630. bioRxiv. 2024. PMID: 38854146 Free PMC article. Preprint.
-
Vascular organoids: unveiling advantages, applications, challenges, and disease modelling strategies.Stem Cell Res Ther. 2023 Oct 10;14(1):292. doi: 10.1186/s13287-023-03521-2. Stem Cell Res Ther. 2023. PMID: 37817281 Free PMC article. Review.
-
Organoids: Construction and Application in Gastric Cancer.Biomolecules. 2023 May 22;13(5):875. doi: 10.3390/biom13050875. Biomolecules. 2023. PMID: 37238742 Free PMC article. Review.
-
Patient-Derived Xenograft: A More Standard "Avatar" Model in Preclinical Studies of Gastric Cancer.Front Oncol. 2022 May 19;12:898563. doi: 10.3389/fonc.2022.898563. eCollection 2022. Front Oncol. 2022. PMID: 35664756 Free PMC article. Review.
References
-
- Agarwal S., Holton K.L., Lanza R. (2008). Efficient differentiation of functional hepatocytes from human embryonic stem cells. Stem Cells 26, 1117–1127. - PubMed
-
- Baharvand H., Hashemi S.M., Kazemi Ashtiani S., et al. (2006). Differentiation of human embryonic stem cells into hepatocytes in 2D and 3D culture systems in vitro. Int. J. Dev. Biol. 50, 645–652. - PubMed