Protective Effects of Chrysin Against Oxidative Stress and Inflammation Induced by Lead Acetate in Rat Kidneys: a Biochemical and Histopathological Approach

doi:10.1007/s12011-020-02268-8

. 2021 Apr;199(4):1501-1514.

doi: 10.1007/s12011-020-02268-8. Epub 2020 Jun 29.

Protective Effects of Chrysin Against Oxidative Stress and Inflammation Induced by Lead Acetate in Rat Kidneys: a Biochemical and Histopathological Approach

Sefa Kucukler¹, Fulya Benzer², Serkan Yildirim³, Cihan Gur¹, Fatih Mehmet Kandemir⁴, Aydin Sukru Bengu⁵, Adnan Ayna⁶, Cuneyt Caglayan⁷, Muhammet Bahaeddin Dortbudak³

Affiliations

¹ Department of Biochemistry, Faculty of Veterinary Medicine, Ataturk University, 25240, Erzurum, Turkey.
² Department of Midwifery, Faculty of Health Science, Munzur University, 62000, Tunceli, Turkey.
³ Department of Pathology, Faculty of Veterinary Medicine, Ataturk University, 25240, Erzurum, Turkey.
⁴ Department of Biochemistry, Faculty of Veterinary Medicine, Ataturk University, 25240, Erzurum, Turkey. fmehmet.kandemir@atauni.edu.tr.
⁵ Department of Medical Services and Tecniques, Program of Medical Laboratory Tecniques, Bingol University, 12000, Bingöl, Turkey.
⁶ Department of Chemistry, Faculty of Sciences and Arts, Bingol University, 12000, Bingöl, Turkey.
⁷ Department of Biochemistry, Faculty of Veterinary Medicine, Bingol University, 12000, Bingöl, Turkey.

PMID: 32613487
DOI: 10.1007/s12011-020-02268-8

Protective Effects of Chrysin Against Oxidative Stress and Inflammation Induced by Lead Acetate in Rat Kidneys: a Biochemical and Histopathological Approach

Sefa Kucukler et al. Biol Trace Elem Res. 2021 Apr.

. 2021 Apr;199(4):1501-1514.

doi: 10.1007/s12011-020-02268-8. Epub 2020 Jun 29.

Authors

Sefa Kucukler¹, Fulya Benzer², Serkan Yildirim³, Cihan Gur¹, Fatih Mehmet Kandemir⁴, Aydin Sukru Bengu⁵, Adnan Ayna⁶, Cuneyt Caglayan⁷, Muhammet Bahaeddin Dortbudak³

Affiliations

¹ Department of Biochemistry, Faculty of Veterinary Medicine, Ataturk University, 25240, Erzurum, Turkey.
² Department of Midwifery, Faculty of Health Science, Munzur University, 62000, Tunceli, Turkey.
³ Department of Pathology, Faculty of Veterinary Medicine, Ataturk University, 25240, Erzurum, Turkey.
⁴ Department of Biochemistry, Faculty of Veterinary Medicine, Ataturk University, 25240, Erzurum, Turkey. fmehmet.kandemir@atauni.edu.tr.
⁵ Department of Medical Services and Tecniques, Program of Medical Laboratory Tecniques, Bingol University, 12000, Bingöl, Turkey.
⁶ Department of Chemistry, Faculty of Sciences and Arts, Bingol University, 12000, Bingöl, Turkey.
⁷ Department of Biochemistry, Faculty of Veterinary Medicine, Bingol University, 12000, Bingöl, Turkey.

PMID: 32613487
DOI: 10.1007/s12011-020-02268-8

Abstract

In this study, the protective effects of chrysin (CR) on lead acetate (PbAc)-induced renal toxicity in Sprague-Dawley rats were investigated with biochemical, histopathological, and immunohistochemical methods. In the study, rats were given orally at 30 mg/kg/body weight (BW) PbAc after CR of 25 and 50 mg/kg/BW was administered to them orally (a total of 7 administrations for 7 days). The results showed that CR reduced urea and creatinine levels by alleviating PbAc-induced kidney damage. It was determined that CR decreases PbAc-induced lipid peroxidation due to its antioxidant properties and increases catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx) activities, and glutathione (GSH) levels. It was also detected that CR protects DNA from the toxic effects of PbAc and reduces 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels. Biochemical and immunohistochemical findings demonstrated that CR had anti-inflammatory and antiapoptotic effects and reduced nuclear factor kappa-B (NF-κB), interleukin-33 (IL-33), prostaglandin-E2 (PGE-2), tumor necrosis factor-α (TNF-α), p53 levels, and the activities of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), which were increased with PbAc administration. Moreover, CR was found to increase the levels of aquaporin-1 (AQP-1) and nephrine in PbAc-induced kidney tissue. CR decreased the contents of lead (Pb), zinc (Zn), iron (Fe), sodium (Na), and copper (Cu) and increased those of potassium (K) calcium (Ca) in renal tissue. These results indicated that CR considerably alleviates kidney toxicity caused by PbAc.

Keywords: Apoptosis; Chrysin; Inflammation; Lead acetate; Nephrotoxicity; Oxidative stress.

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References

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1. Khalil SR, Khalifa HA, Abdel-Motal SM, Mohammed HH, Elewa YHA, Mahmoud HA (2018) Spirulina platensis attenuates the associated neurobehavioral and inflammatory response impairments in rats exposed to lead acetate. Ecotoxicol Environ Saf 157:255–265. https://doi.org/10.1016/j.ecoenv.2018.03.068 - DOI - PubMed
1. Soleimani E, Goudarzi I, Abrari K, Lashkarbolouki T (2016) The combined effects of developmental lead and ethanol exposure on hippocampus dependent spatial learning and memory in rats: role of oxidative stress. Food Chem Toxicol 96:263–272. https://doi.org/10.1016/j.fct.2016.07.009 - DOI - PubMed
1. Khalil SR, Elhady WM, Elewa YHA, Abd El-Hameed NE, Ali SA (2018) Possible role of Arthrospira platensis in reversing oxidative stress-mediated liver damage in rats exposed to lead. Biomed Pharmacother 97:1259–1268. https://doi.org/10.1016/j.biopha.2017.11.045 - DOI - PubMed
1. Shojaeepour S, Fazeli M, Oghabian Z, Pourgholi L, Mandegary A (2018) Oxidative stress in opium users after using lead-adulterated opium: the role of genetic polymorphism. Food Chem Toxicol 120:571–577. https://doi.org/10.1016/j.fct.2018.07.061 - DOI - PubMed

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[1] Phyu MP, Tangpong J (2014) Neuroprotective effects of xanthone derivative of Garcinia mangostana against lead-induced acetylcholinesterase dysfunction and cognitive impairment. Food Chem Toxicol 70:151–156. https://doi.org/10.1016/j.fct.2014.04.035 - DOI - PubMed

[2] Phyu MP, Tangpong J (2014) Neuroprotective effects of xanthone derivative of Garcinia mangostana against lead-induced acetylcholinesterase dysfunction and cognitive impairment. Food Chem Toxicol 70:151–156. https://doi.org/10.1016/j.fct.2014.04.035 - DOI - PubMed

[3] Khalil SR, Khalifa HA, Abdel-Motal SM, Mohammed HH, Elewa YHA, Mahmoud HA (2018) Spirulina platensis attenuates the associated neurobehavioral and inflammatory response impairments in rats exposed to lead acetate. Ecotoxicol Environ Saf 157:255–265. https://doi.org/10.1016/j.ecoenv.2018.03.068 - DOI - PubMed

[4] Khalil SR, Khalifa HA, Abdel-Motal SM, Mohammed HH, Elewa YHA, Mahmoud HA (2018) Spirulina platensis attenuates the associated neurobehavioral and inflammatory response impairments in rats exposed to lead acetate. Ecotoxicol Environ Saf 157:255–265. https://doi.org/10.1016/j.ecoenv.2018.03.068 - DOI - PubMed

[5] Soleimani E, Goudarzi I, Abrari K, Lashkarbolouki T (2016) The combined effects of developmental lead and ethanol exposure on hippocampus dependent spatial learning and memory in rats: role of oxidative stress. Food Chem Toxicol 96:263–272. https://doi.org/10.1016/j.fct.2016.07.009 - DOI - PubMed

[6] Soleimani E, Goudarzi I, Abrari K, Lashkarbolouki T (2016) The combined effects of developmental lead and ethanol exposure on hippocampus dependent spatial learning and memory in rats: role of oxidative stress. Food Chem Toxicol 96:263–272. https://doi.org/10.1016/j.fct.2016.07.009 - DOI - PubMed

[7] Khalil SR, Elhady WM, Elewa YHA, Abd El-Hameed NE, Ali SA (2018) Possible role of Arthrospira platensis in reversing oxidative stress-mediated liver damage in rats exposed to lead. Biomed Pharmacother 97:1259–1268. https://doi.org/10.1016/j.biopha.2017.11.045 - DOI - PubMed

[8] Khalil SR, Elhady WM, Elewa YHA, Abd El-Hameed NE, Ali SA (2018) Possible role of Arthrospira platensis in reversing oxidative stress-mediated liver damage in rats exposed to lead. Biomed Pharmacother 97:1259–1268. https://doi.org/10.1016/j.biopha.2017.11.045 - DOI - PubMed

[9] Shojaeepour S, Fazeli M, Oghabian Z, Pourgholi L, Mandegary A (2018) Oxidative stress in opium users after using lead-adulterated opium: the role of genetic polymorphism. Food Chem Toxicol 120:571–577. https://doi.org/10.1016/j.fct.2018.07.061 - DOI - PubMed

[10] Shojaeepour S, Fazeli M, Oghabian Z, Pourgholi L, Mandegary A (2018) Oxidative stress in opium users after using lead-adulterated opium: the role of genetic polymorphism. Food Chem Toxicol 120:571–577. https://doi.org/10.1016/j.fct.2018.07.061 - DOI - PubMed

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Protective Effects of Chrysin Against Oxidative Stress and Inflammation Induced by Lead Acetate in Rat Kidneys: a Biochemical and Histopathological Approach

Affiliations

Protective Effects of Chrysin Against Oxidative Stress and Inflammation Induced by Lead Acetate in Rat Kidneys: a Biochemical and Histopathological Approach

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