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. 2020 Jun 6:2020:6942740.
doi: 10.1155/2020/6942740. eCollection 2020.

Effect of Different Expression of Immune-Related lncRNA on Colon Adenocarcinoma and Its Relation to Prognosis

Meiwei Mu  1 , Yi Tang  2 , Zheng Yang  1 , Yuling Qiu  1 , Xiaohong Li  1 , Wuning Mo  1 , Qisheng Su  1
Affiliations

Effect of Different Expression of Immune-Related lncRNA on Colon Adenocarcinoma and Its Relation to Prognosis

Meiwei Mu et al. Biomed Res Int. .

Abstract

Objective: To explore the expression of immune-related lncRNAs in colon adenocarcinoma and find out the effect on how these lncRNAs influence the development and prognosis of colon adenocarcinoma.

Method: Transcriptome data of colon adenocarcinoma from The Cancer Genome Atlas (TCGA) were downloaded, and gene sets "IMMUNE RESPONSE" and "IMMUNE SYSTEM PROCESS" were sought from the Molecular Signatures Database (MSigDB). The expression of immune-related genes was extracted that were immune-related mRNAs. Then, the immune-related lncRNAs were sought out by utilizing of the above data. Clinical traits were combined with immune-related lncRNAs, so that prognostic-related lncRNAs were identified by Cox regression. Multivariate Cox regression was built to calculate risk scores. Relationships between clinical traits and immune-related lncRNAs were also calculated.

Result: A total of 480 colorectal adenocarcinoma patients and 41 normal control patients' transcriptome sequencing data of tissue samples were obtained from TCGA database. 918 immune-related lncRNAs were screened. Cox regression showed that 34 immune-related lncRNAs were associated with colon adenocarcinoma prognosis. Seven lncRNAs were independent risk factors.

Conclusion: This study revealed that some lncRNAs can affect the development and prognosis of colon adenocarcinoma. It may provide new theory evidence of molecular mechanism for the future research and molecular targeted therapy of colon adenocarcinoma.

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Conflict of interest statement

The authors declare that they have no conflicts of interest.

Figures

Figure 1
Figure 1
Univariate Cox regression showed that 34 immune-related lncRNAs were risk factors for COAD patients (hazard ratio > 1).
Figure 2
Figure 2
The survival curve shows that patients in the high-risk score group have a shorter survival time than those in the low-risk score group (a). The heat map of prognosis-related lncRNAs in COAD (b). Risk curves were drawn based on the data of 480 patients. Take the risk scores as the vertical coordinate and the patient data as the horizontal coordinate. We can see the risk scores were increasing, in which the low-risk curve was in green whereas the high-risk curve was in red (c). Green dots mean people who were still alive, and red dots mean people who were already dead. As the risk scores were increasing, the deaths were added; the red dots were concentrated in the high-risk area. And in the low-risk area, where the green dots were concentrated, patients are almost still alive (d).
Figure 3
Figure 3
Univariate Cox regression showed that age, clinical stage, T stage, N stage, M stage, and risk score were all correlated with the prognosis of COAD patients (a). Multivariate Cox regression showed that only the risk score was an independent risk factor for COAD (b), and the ROC curve showed that the risk score had the largest area under the curve (c).
Figure 4
Figure 4
Multiple immune-related lncRNAs were associated with clinical traits (∗ means they had relevance, and the more they had, the greater the correlation; “ns” means they had no relevance). (a) Relationship between immune-related lncRNAs and clinical stages. (b) Relationship between immune-related lncRNAs and T stages. (c) Relationship between immune-related lncRNAs and N stages. (d) Relationship between immune-related lncRNAs and M stages.
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