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. 2020 Aug;37(4):423-433.
doi: 10.1007/s10719-020-09927-x. Epub 2020 Jun 24.

The expression and functional analysis of the sialyl-T antigen in prostate cancer

Ruifeng Bai  1 , Xue Luan  1 , Yu Zhang  1 , Catherine Robbe-Masselot  2 , Inka Brockhausen  3 , Yin Gao  4   5
Affiliations

The expression and functional analysis of the sialyl-T antigen in prostate cancer

Ruifeng Bai et al. Glycoconj J. 2020 Aug.

Abstract

Aberrant glycosylation is a featured characteristic of cancer and plays a role in cancer pathology; thus an understanding of the compositions and functions of glycans is critical for discovering diagnostic biomarkers and therapeutic targets for cancer. In this study, we used MALDI-TOF-MS analysis to determine the O-glycan profiles of prostate cancer cells metastasized to bone (PC-3), brain (DU145), lymph node (LNCaP), and vertebra (VCaP) in comparison to immortalized RWPE-1 cells derived from normal prostatic tissue. Prostate cancer (CaP) cells exhibited an elevation of simple/short O-glycans, with a reduction of complex O-glycans, increased O-glycan sialylation and decreased fucosylation. Core 1 sialylation was increased dramatically in all CaP cells, and especially in PC-3 cells. The expression of Neu5Acα2-3Galβ1-3GalNAc- (sialyl-3T antigen) which is the product of α2,3-sialyltransferase-I (ST3Gal-I) was substantially increased. We therefore focused on exploring the possible function of ST3Gal-I in PC-3 cells. ST3Gal-I silencing studies showed that ST3Gal-I was associated with PC-3 cell proliferation, migration and apoptosis. Further in vivo studies demonstrated that down regulation of ST3Gal-I reduced the tumor size in xenograft mouse model, indicating that sialyl-3T can serve as a biomarker for metastatic prostate cancer prognosis, and that ST3Gal-I could be a target for therapeutic intervention in cancer treatment.

Keywords: Glycosylation; Mass spectrometry; Prostate cancer; Sialyl-3T antigen; α2,3-sialyltransferase.

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