Single-cell landscape of bronchoalveolar immune cells in patients with COVID-19

doi:10.1038/s41591-020-0901-9

. 2020 Jun;26(6):842-844.

doi: 10.1038/s41591-020-0901-9. Epub 2020 May 12.

Single-cell landscape of bronchoalveolar immune cells in patients with COVID-19

Mingfeng Liao^#¹, Yang Liu^#¹, Jing Yuan^#², Yanling Wen¹, Gang Xu¹, Juanjuan Zhao¹, Lin Cheng¹, Jinxiu Li², Xin Wang¹, Fuxiang Wang², Lei Liu^{3

4}, Ido Amit⁵, Shuye Zhang⁶, Zheng Zhang^{7

8}

Affiliations

¹ Institute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People's Hospital, Shenzhen, China.
² Department for Infectious Diseases, Shenzhen Third People's Hospital, Shenzhen, China.
³ Institute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People's Hospital, Shenzhen, China. liulei3322@aliyun.com.
⁴ The Second Affiliated Hospital, School of Medicine, Southern University of Science and Technology, Shenzhen, China. liulei3322@aliyun.com.
⁵ Department of Immunology, Weizmann Institute of Science, Rehovot, Israel. ido.amit@weizmann.ac.il.
⁶ Shanghai Public Health Clinical Center, Fudan University, Shanghai, China. zhangshuye@shphc.org.cn.
⁷ Institute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People's Hospital, Shenzhen, China. zhangzheng1975@aliyun.com.
⁸ The Second Affiliated Hospital, School of Medicine, Southern University of Science and Technology, Shenzhen, China. zhangzheng1975@aliyun.com.

^# Contributed equally.

PMID: 32398875
DOI: 10.1038/s41591-020-0901-9

Single-cell landscape of bronchoalveolar immune cells in patients with COVID-19

Mingfeng Liao et al. Nat Med. 2020 Jun.

. 2020 Jun;26(6):842-844.

doi: 10.1038/s41591-020-0901-9. Epub 2020 May 12.

Authors

Affiliations

¹ Institute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People's Hospital, Shenzhen, China.
² Department for Infectious Diseases, Shenzhen Third People's Hospital, Shenzhen, China.
³ Institute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People's Hospital, Shenzhen, China. liulei3322@aliyun.com.
⁴ The Second Affiliated Hospital, School of Medicine, Southern University of Science and Technology, Shenzhen, China. liulei3322@aliyun.com.
⁵ Department of Immunology, Weizmann Institute of Science, Rehovot, Israel. ido.amit@weizmann.ac.il.
⁶ Shanghai Public Health Clinical Center, Fudan University, Shanghai, China. zhangshuye@shphc.org.cn.
⁷ Institute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People's Hospital, Shenzhen, China. zhangzheng1975@aliyun.com.
⁸ The Second Affiliated Hospital, School of Medicine, Southern University of Science and Technology, Shenzhen, China. zhangzheng1975@aliyun.com.

^# Contributed equally.

PMID: 32398875
DOI: 10.1038/s41591-020-0901-9

Abstract

Respiratory immune characteristics associated with Coronavirus Disease 2019 (COVID-19) severity are currently unclear. We characterized bronchoalveolar lavage fluid immune cells from patients with varying severity of COVID-19 and from healthy people by using single-cell RNA sequencing. Proinflammatory monocyte-derived macrophages were abundant in the bronchoalveolar lavage fluid from patients with severe COVID-9. Moderate cases were characterized by the presence of highly clonally expanded CD8⁺ T cells. This atlas of the bronchoalveolar immune microenvironment suggests potential mechanisms underlying pathogenesis and recovery in COVID-19.

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Single-cell analysis reveals bronchoalveolar epithelial dysfunction in COVID-19 patients.
He J, Cai S, Feng H, Cai B, Lin L, Mai Y, Fan Y, Zhu A, Huang H, Shi J, Li D, Wei Y, Li Y, Zhao Y, Pan Y, Liu H, Mo X, He X, Cao S, Hu F, Zhao J, Wang J, Zhong N, Chen X, Deng X, Chen J. He J, et al. Protein Cell. 2020 Sep;11(9):680-687. doi: 10.1007/s13238-020-00752-4. Protein Cell. 2020. PMID: 32671793 Free PMC article. No abstract available.

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References

1. Channappanavar, R. et al. Dysregulated type I interferon and inflammatory monocyte-macrophage responses cause lethal pneumonia in SARS-CoV-Infected mice. Cell Host Microbe 19, 181–193 (2016). - DOI
1. Liu, L. et al. Anti–spike IgG causes severe acute lung injury by skewing macrophage responses during acute SARS-CoV infection. JCI Insight 4, e123158 (2019). - DOI
1. Cao, X. COVID-19: immunopathology and its implications for therapy. Nat. Rev. Immunol. 20, 269–270 (2020). - DOI
1. Morse, C. et al. Proliferating SPP1/MERTK-expressing macrophages in idiopathic pulmonary fibrosis. Eur. Respir. J. 54, 1802441 (2019). - DOI
1. Reyfman, P. A. et al. Single-cell transcriptomic analysis of human lung provides insights into the pathobiology of pulmonary fibrosis. Am. J. Respir. Crit. Care Med. 199, 1517–1536 (2019). - DOI

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[1] Channappanavar, R. et al. Dysregulated type I interferon and inflammatory monocyte-macrophage responses cause lethal pneumonia in SARS-CoV-Infected mice. Cell Host Microbe 19, 181–193 (2016). - DOI

[2] Channappanavar, R. et al. Dysregulated type I interferon and inflammatory monocyte-macrophage responses cause lethal pneumonia in SARS-CoV-Infected mice. Cell Host Microbe 19, 181–193 (2016). - DOI

[3] Liu, L. et al. Anti–spike IgG causes severe acute lung injury by skewing macrophage responses during acute SARS-CoV infection. JCI Insight 4, e123158 (2019). - DOI

[4] Liu, L. et al. Anti–spike IgG causes severe acute lung injury by skewing macrophage responses during acute SARS-CoV infection. JCI Insight 4, e123158 (2019). - DOI

[5] Cao, X. COVID-19: immunopathology and its implications for therapy. Nat. Rev. Immunol. 20, 269–270 (2020). - DOI

[6] Cao, X. COVID-19: immunopathology and its implications for therapy. Nat. Rev. Immunol. 20, 269–270 (2020). - DOI

[7] Morse, C. et al. Proliferating SPP1/MERTK-expressing macrophages in idiopathic pulmonary fibrosis. Eur. Respir. J. 54, 1802441 (2019). - DOI

[8] Morse, C. et al. Proliferating SPP1/MERTK-expressing macrophages in idiopathic pulmonary fibrosis. Eur. Respir. J. 54, 1802441 (2019). - DOI

[9] Reyfman, P. A. et al. Single-cell transcriptomic analysis of human lung provides insights into the pathobiology of pulmonary fibrosis. Am. J. Respir. Crit. Care Med. 199, 1517–1536 (2019). - DOI

[10] Reyfman, P. A. et al. Single-cell transcriptomic analysis of human lung provides insights into the pathobiology of pulmonary fibrosis. Am. J. Respir. Crit. Care Med. 199, 1517–1536 (2019). - DOI

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Single-cell landscape of bronchoalveolar immune cells in patients with COVID-19

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Single-cell landscape of bronchoalveolar immune cells in patients with COVID-19

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