Overview of multiplex immunohistochemistry/immunofluorescence techniques in the era of cancer immunotherapy
- PMID: 32301585
- PMCID: PMC7170662
- DOI: 10.1002/cac2.12023
Overview of multiplex immunohistochemistry/immunofluorescence techniques in the era of cancer immunotherapy
Abstract
Conventional immunohistochemistry (IHC) is a widely used diagnostic technique in tissue pathology. However, this technique is associated with a number of limitations, including high inter-observer variability and the capacity to label only one marker per tissue section. This review details various highly multiplexed techniques that have emerged to circumvent these constraints, allowing simultaneous detection of multiple markers on a single tissue section and the comprehensive study of cell composition, cellular functional and cell-cell interactions. Among these techniques, multiplex Immunohistochemistry/Immunofluorescence (mIHC/IF) has emerged to be particularly promising. mIHC/IF provides high-throughput multiplex staining and standardized quantitative analysis for highly reproducible, efficient and cost-effective tissue studies. This technique has immediate potential for translational research and clinical practice, particularly in the era of cancer immunotherapy.
Keywords: immunofluorescence; immunohistochemistry; immunotherapy; multiplex; overview.
© 2020 The Authors. Cancer Communications published by John Wiley & Sons Australia, Ltd. on behalf of Sun Yat-sen University Cancer Center.
Conflict of interest statement
The authors declare that they have no competing interests.
Figures
Similar articles
-
[Overview of Multiplex Immunohistochemistry and Immunofluorescence Techniques in the Lung Cancer Immunotherapy].Zhongguo Fei Ai Za Zhi. 2021 Jan 20;24(1):36-42. doi: 10.3779/j.issn.1009-3419.2020.102.47. Zhongguo Fei Ai Za Zhi. 2021. PMID: 33478189 Free PMC article. Review. Chinese.
-
The Society for Immunotherapy of Cancer statement on best practices for multiplex immunohistochemistry (IHC) and immunofluorescence (IF) staining and validation.J Immunother Cancer. 2020 May;8(1):e000155. doi: 10.1136/jitc-2019-000155. J Immunother Cancer. 2020. PMID: 32414858 Free PMC article.
-
Multiplex Immunofluorescence: A Powerful Tool in Cancer Immunotherapy.Int J Mol Sci. 2023 Feb 4;24(4):3086. doi: 10.3390/ijms24043086. Int J Mol Sci. 2023. PMID: 36834500 Free PMC article. Review.
-
Multiplex Immunohistochemistry and Immunofluorescence: A Practical Update for Pathologists.Mod Pathol. 2023 Jul;36(7):100197. doi: 10.1016/j.modpat.2023.100197. Epub 2023 Apr 25. Mod Pathol. 2023. PMID: 37105494 Review.
-
Multiplex immunohistochemistry/immunofluorescence (mIHC/IF) for PD-L1 testing in triple-negative breast cancer: a translational assay compared with conventional IHC.J Clin Pathol. 2020 Sep;73(9):557-562. doi: 10.1136/jclinpath-2019-206252. Epub 2020 Jan 22. J Clin Pathol. 2020. PMID: 31969377
Cited by
-
Spatial proteomics: unveiling the multidimensional landscape of protein localization in human diseases.Proteome Sci. 2024 Sep 20;22(1):7. doi: 10.1186/s12953-024-00231-2. Proteome Sci. 2024. PMID: 39304896 Free PMC article. Review.
-
PUPAID: A R + ImageJ pipeline for thorough and semi-automated processing and analysis of multi-channel immunofluorescence data.PLoS One. 2024 Sep 19;19(9):e0308970. doi: 10.1371/journal.pone.0308970. eCollection 2024. PLoS One. 2024. PMID: 39298534 Free PMC article.
-
The role of HGH1 in breast cancer prognosis: a study on immune response and cell cycle.BMC Cancer. 2024 Sep 9;24(1):1122. doi: 10.1186/s12885-024-12879-2. BMC Cancer. 2024. PMID: 39251967 Free PMC article.
-
Deciphering the Role of BRAFV600E Immunohistochemistry in Breast Lesions: A Comprehensive Review.Cureus. 2024 Jul 18;16(7):e64872. doi: 10.7759/cureus.64872. eCollection 2024 Jul. Cureus. 2024. PMID: 39156294 Free PMC article. Review.
-
Fluorescence Lifetime Multiplexing (FLEX) for simultaneous high dimensional spatial biology in 3D.Commun Biol. 2024 Aug 18;7(1):1012. doi: 10.1038/s42003-024-06702-8. Commun Biol. 2024. PMID: 39154126 Free PMC article.
References
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous