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Case Reports
. 2020 Mar 5;82(3):294-298.
doi: 10.1292/jvms.19-0615. Epub 2020 Jan 13.

Multicystic peritoneal tumor in two layer hens

Keiko Yoshida  1 , Tomoyuki Miyoshi  2 , Tomoaki Murakami  2
Affiliations
Case Reports

Multicystic peritoneal tumor in two layer hens

Keiko Yoshida et al. J Vet Med Sci. .

Abstract

In chicken, peritoneal cystic lesions have not been clearly categorized. In this study, diffuse peritoneal multiple cysts were observed in two layer hens. The cysts in the serosa were lined with single layers of squamous or cuboidal cells. The papillary proliferations of columnar cells were also observed in one case. The smooth muscle layer or mass were observed around the cysts in both cases. The cystic lining cells were positive for pan-cytokeratin, vimentin, S100 and Wilms tumor 1. Ultrastructurally, they had sparsely microvilli on the luminal surface. The histological results indicated the present cases were multicystic mesothelioma, but also had characteristics of Mullerian epithelium. This is the first report describing the detailed pathological feature of unique multicystic tumor in chicken.

Keywords: chicken; electron microscopy; immunohistochemistry; mesothelioma; peritoneal cyst.

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Figures

Fig. 1.
Fig. 1.
Macroscopic feature of the gastrointestinal tract of case 1. There were multiple transparent cysts on the peritoneum, especially around the duodenum, pancreas and mesentery. a, proventriculus; b, gizzard; c, pancreas. Bar, 3 cm.
Fig. 2.
Fig. 2.
Histological feature of cystic lesion. a: The neoplastic cells formed the cysts and papillary proliferation (arrow). Calcification (arrowhead) was occasionally observed in the stroma of papillary lesions. Case 1. H&E stain. Bar, 200 µm. b: Proliferation of spindle cells was observed in the stroma of cysts (arrow). Multifocal or diffuse mononuclear cell infiltration was also observed in the stroma. Case 2. H&E stain. Bar, 200 µm. c: PAS-positive amorphous deposits (arrows) were observed in the stroma of cysts. Case 2. PAS stain. Bar, 100 µm. d: Calcified circle structures in the stroma of papillary lesions were positive for PAS. Case 1. PAS stain. Bar, 100 µm. Insert, von Kossa stain.
Fig. 3.
Fig. 3.
Immunohistochemistry of cystic lesion. a–e: The neoplastic cells were strongly positive for CK (a) and vimentin (b) in the cytoplasm; mildly positive for S100 in the cytoplasm and the nucleus (c); strongly positive for calretinin on the luminal surface, but negative in the nucleus (d); partially positive for WT1 in the nucleus (e). Inserts, high magnification. Case 1. Bars, 100 µm. f: The layer and mass of spindle cells surrounding the cysts were positive for SMA. Case 2. Bar, 100 µm.
Fig. 4.
Fig. 4.
Ultrastructure of the neoplastic cells in case 2. They had sparsely microvilli (arrows) on the luminal surface. The cell membranes were closely aligned with interdigitations. Bar, 5 µm.
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References

    1. Apperson K. D., Bird K. E., Cherian G., Löhr C. V.2017. Histology of the ovary of the laying hen (Gallus domesticus). Vet Sci 4: E66. doi: 10.3390/vetsci4040066 - DOI - PMC - PubMed
    1. Attanoos R. L., Webb R., Dojcinov S. D., Gibbs A. R.2002. Value of mesothelial and epithelial antibodies in distinguishing diffuse peritoneal mesothelioma in females from serous papillary carcinoma of the ovary and peritoneum. Histopathology 40: 237–244. doi: 10.1046/j.1365-2559.2002.01352.x - DOI - PubMed
    1. August C. Z., Murad T. M., Newton M.1985. Multiple focal extraovarian serous carcinoma. Int. J. Gynecol. Pathol. 4: 11–23. doi: 10.1097/00004347-198501000-00002 - DOI - PubMed
    1. Carlson J. W., Miron A., Jarboe E. A., Parast M. M., Hirsch M. S., Lee Y., Muto M. G., Kindelberger D., Crum C. P.2008. Serous tubal intraepithelial carcinoma: its potential role in primary peritoneal serous carcinoma and serous cancer prevention. J. Clin. Oncol. 26: 4160–4165. doi: 10.1200/JCO.2008.16.4814 - DOI - PMC - PubMed
    1. Chabot J. F., Beard D., Langlois A. J., Beard J. W.1970. Mesotheliomas of peritoneum, epicardium, and pericardium induced by strain MC29 avian leukosis virus. Cancer Res. 30: 1287–1308. - PubMed

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