Skip to main page content
U.S. flag

An official website of the United States government

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019 Nov;44(6):501-510.
doi: 10.30476/ijms.2019.44961.

The Healing Effects of Hydroalcoholic Extract of Carum copticum L. on Experimental Colitis in Rats

Donya Firoozi  1 , Ali Akbar Nekooeian  2 , Nader Tanideh  2 , Zohreh Mazloom  3 , Maral Mokhtari  4 , Mohsen Mohammadi Sartang  5
Affiliations

The Healing Effects of Hydroalcoholic Extract of Carum copticum L. on Experimental Colitis in Rats

Donya Firoozi et al. Iran J Med Sci. 2019 Nov.

Abstract

Background: Ulcerative colitis is an inflammatory disease with indefinite treatment. The present study aimed to assess the anti-inflammatory and antioxidant effects of Carum copticum L. (CC) extract on induced colitis in rats.

Methods: Sixty male rats were randomly divided into six groups (n=10 per group). Acetic acid-induced colitis rats were orally administered with doses of 100, 200, and 400 mg/kg CC extract, and 100 mg/kg sulfasalazine for seven consecutive days, respectively. Colonic biopsies were taken to measure histopathological parameters as well as the tumor necrosis factor (TNF-α), interleukin-6 (IL-6), myeloperoxidase (MPO), malondialdehyde (MDA), and glutathione (GSH). Data analysis was performed using the one-way ANOVA and Tukey's test for normally distributed data. Kruskal-Wallis test followed by Dunn's test was used for non-normally distributed data. The analysis was performed at P≤0.05 using SigmaStat software (version 10.0).

Results: The control colitis group had a significantly higher total colitis index (P=0.01), TNF-α (P=0.01), IL-6 (P=0.01), MPO (P=0.01), and MDA (P=0.01); and lower GSH (P=0.01) than those of the sham group. The colitis group receiving a dose of 200 mg/kg/day CC extract had a significantly lower total colitis index (P=0.01), TNF-α (P=0.01), IL-6 (P=0.01), MPO (P=0.01), and MDA (P=0.01); and higher GSH (P=0.01) than those of the control colitis group. The colitis group receiving a dose of 200 mg/kg/day CC extract had a significantly lower total colitis index (P=0.04), TNF-α (P=0.03), IL-6 (P=0.04), MPO (P=0.03), and MDA (P=0.03); and higher GSH (P=0.01) than those of the colitis group receiving sulfasalazine.

Conclusion: The present study revealed that CC extract had healing effects on colitis, possibly due to its antioxidant and anti-inflammatory properties.

Keywords: Anti-Inflammatory agents; Antioxidants; Cytokines; Rats; Ulcerative; Colitis.

PubMed Disclaimer

Figures

Figure1
Figure1
Microscopic images (H&E, ×400) of colonic tissues from colitis groups receiving vehicle. It shows extensive damage of (A1) crypt abscess, (A2) crypt damage and goblet cell depletion, and (A3) cryptitis. (B): Hydroalcoholic extract of CC (100 mg/kg) shows mild regeneration of colon with necrosis, ulceration, and inflammation.
Figure2
Figure2
Shows the colonic tissue levels (mean±SEM, n=10 each) of TNF-a, IL-6 and MPO. Sham: Group treated with vehicle, Colitis control: Groups treated with vehicle, Colitis-E1: 100 mg/kg/day CC hydroalcoholic extract, Colitis-E2: 200 mg/kg/day CC hydroalcoholic extract, Colitis-E3: 400 mg/kg/day CC hydroalcoholic extract, Colitis-SUL: 100 mg/kg/day sulfasalazine; *Significant different from the sham group; DSignificant difference from the control colitis group; +Significant difference from the sulfasalazine-treated colitis group
Figure3
Figure3
Shows colonic tissue levels (mean±SEM, n=8-10 each) of MDA (A), and GSH peroxidase (B). Sham: Group treated with vehicle, Colitis control: Groups treated with vehicle, Colitis-E1: 100 mg/kg/day CC hydroalcoholic extract, Colitis-E2: 200 mg/kg/day CC hydroalcoholic extract, Colitis-E3: 400 mg/kg/day CC hydroalcoholic extract, Colitis-SUL: 100 mg/kg/day sulfasalazine; *Significant different from the sham group; DSignificant difference from control colitis group; +Significant difference from sulfasalazine-treated colitis group
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Sartor RB. Mechanisms of disease: pathogenesis of Crohn’s disease and ulcerative colitis. Nat Clin Pract Gastroenterol Hepatol. 2006;3:390–407. doi: 10.1038/ncpgasthep0528. - DOI - PubMed
    1. Molodecky NA, Soon IS, Rabi DM, Ghali WA, Ferris M, Chernoff G, et al. Increasing incidence and prevalence of the inflammatory bowel diseases with time, based on systematic review. Gastroenterology. 2012;142:46–54. doi: 10.1053/j.gastro.2011.10.001. - DOI - PubMed
    1. Mashtoub S, Hoang BV, Vu M, Lymn KA, Feinle-Bisset C, Howarth GS. Clinical and structural effects of traditional Chinese medicine and the herbal preparation, Iberogast, in a rat model of ulcerative colitis. J Evid Based Complementary Altern Med. 2014;19:10–9. doi: 10.1177/2156587213503660. - DOI - PubMed
    1. Mathew MM, Han NV, Murugesan A, Raj EA, Prasanth KG. Evaluation of the protective effect of Pterocarpus marsupium on acetic acid-induced ulcerative colitis in rats. Inflammopharmacology. 2015;23:195–201. doi: 10.1007/s10787-015-0234-3. - DOI - PubMed
    1. Ponder A, Long MD. A clinical review of recent findings in the epidemiology of inflammatory bowel disease. Clin Epidemiol. 2013;5:237–47. doi: 10.2147/CLEP.S33961. [ PMC Free Article] - DOI - PMC - PubMed

LinkOut - more resources